| 笃斯越桔果实总DNA提取方法的比较研究 |
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| 引用本文： | 王程,罗军,田志杰,姜艳霞,李妍,许娜,芦晓晶,吕士杰.笃斯越桔果实总DNA提取方法的比较研究[J].吉林医药学院学报,2009,30(1). |
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| 作者姓名： | 王程 罗军 田志杰 姜艳霞 李妍 许娜 芦晓晶 吕士杰 |
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| 作者单位： | 吉林医药学院生物化学教研室,吉林,吉林,132013 |
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| 基金项目： | 吉林省教育厅科学技术研究基金 |
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| 摘 要： | 目的探讨越桔果汁总DNA的最佳提取方法。方法分别采用SDS法、高盐低pH值法、CTAB法、异硫氰酸胍法提取越桔果汁的基因组DNA,并对其进行核酸含量测定和电泳及RAPD-PCR检测。结果SDS法、高盐低pH值法、CTAB法、异硫氰酸胍法提取越桔果汁的基因组DNA其OD260/OD280分别为1.944、1.738、1.661、1.813,其浓度分别为2.363μg/μl、0.548μg/μl、0.735μg/μl、1.455μg/μl;电泳检测显示异硫氰酸胍法提取DNA条带最清晰,扩增产物最多。结论用异硫氰酸胍法提取越桔果汁总DNA优于其它方法。
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| 关 键 词： | 越桔 DNA提取 SDS法 高盐低pH值法 CTAB法 异硫氰酸胍法 |
Comparative study on extracting DNA from blueberry |
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| WANG Cheng,LUO Jun,TIAN Zhi-jie,JIANG Yan-xia,LI Yan,XU Na,LU Xiao-jing,Lü Shi-jie.Comparative study on extracting DNA from blueberry[J].Journal of Jilin Medical College,2009,30(1). |
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| Authors: | WANG Cheng LUO Jun TIAN Zhi-jie JIANG Yan-xia LI Yan XU Na LU Xiao-jing Lü Shi-jie |
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| Institution: | Department of Biochemistry;Jilin Medical College;Jilin City;Jilin Province;132013;China |
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| Abstract: | Objective To acquire an efficient method of extracting DNA from processed blueberry juice.Methods DNA was extracted by SDS,High salty low pH value buffer,CTAB and Guanidinium thiocyanate respectively,the quality and quantity of extracted DNA were analysed by spectrophotometer,electrophoretic and RAPD-PCR.Results OD260/OD280 of blueberry juice genome DNA respectively extracted by SDS,High salty pH value buffer,CTAB and Guanidinium thiocyanate extraction was 1.944,1.738,1.661,1.813,the density was 2.363 μg/μl... |
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| Keywords: | blueberry DNA extraction SDS High salty low pH value buffer CTAB guanidinium thiocyanate |
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