多巴胺能系统亢进法建立拟抽动秽语综合征小鼠模型

目的 经多巴胺能系统亢进的方法建立并评价拟抽动秽语综合征小鼠模型。方法 经硫酸苯丙胺 3mg/kg单次腹腔注射和阿朴吗啡 2mg/kg单次皮下注射建立小鼠模型。经攀爬行为、自主活动次数以及纹状体内单胺类神经递质及其代谢产物含量的测定对两种小鼠模型加以检测和评价。结果 苯丙胺模型组小鼠 5min内的自主活动次数为 237. 07,较对照组增加 51. 04%,纹状体内多巴胺含量较对照组升高 28. 33%;阿朴吗啡可引起模型小鼠的特殊攀爬行为,在各测定时段内的握杆时间均较对照组明显延长。结论 苯丙胺小鼠模型和阿朴吗啡小鼠模型都能够部分模拟临床抽动秽语综合征病人的神经生化或行为学改变,二者可以作为药物初步筛选的平台。

Development of Tourette syndrome mice model by dopaminergic pathway

Objective To develop animal models to mimic the behavioral or neurochemical changes of Tourette Syndrome (TS) by dopaminergic pathway. Methods TS animal models were developed by intraperitoneal injection of amphetamine or subcutaneous injection of apomorphine. The special climbing behavior was recorded.Computer was used to record the spontaneous movements of AMP mice and HPLC (high performance of liquid chromatography) was used to detect the content of dopamine in striatum. ResultsAverage spontaneous movements of AMP model mice in 5 minutes were 237.07 and increased 51.04% compared with control group. The content of dopamine in striatum was increased 28.33% compared with control group. APO model mice exhibited a special climbing behavior. Grasping time of model mice increased in each measuring course. ConclusionAMP and APO model mice can mimic the neurochemical or behavioral changes of TS partially; they can both be taken as the platform for drug screening.