| 黄芪总苷对人肺腺癌A549细胞增殖及凋亡的影响 |
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| 引用本文： | 牛艳,张羽飞. 黄芪总苷对人肺腺癌A549细胞增殖及凋亡的影响[J]. 牡丹江医学院学报, 2008, 29(3): 16-18 |
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| 作者姓名： | 牛艳 张羽飞 |
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| 作者单位： | 牡丹江天利医药有限公司,黑龙江,牡丹江,157000;牡丹江医学院抗纤维化生物治疗重点实验室,黑龙江,牡丹江,157000 |
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| 摘 要： | 目的:研究黄芪总苷(TA)对人肺腺癌A549细胞增值及凋亡的影响.方法:采用不同药物浓度作用于人肺腺癌A549细胞,应用MTT、DNA琼脂糖凝胶电泳、流式细胞术分析药物对细胞的影响.结果:TA对A549细胞有明显的生长抑制及凋亡作用,并呈剂量、时间依赖性.DNA凝胶电泳显示出特征性凋亡梯状带.结论:在一定条件下,TA可抑制人肺腺癌细胞增殖,抑制作用具有明显的浓度和时间依赖性,同时可诱导其凋亡,凋亡可能与细胞周期阻滞有关.
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| 关 键 词： | 黄芪总苷 人肺腺癌A549细胞 增殖 凋亡 |
EFFECTS OF TOTAL ASTRAGALOSIDES ON PROLIFERATION AND APOPTOSIS OF HUMAN LUNG CANCER CELL LINE A549 |
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| Niu Yan etal. EFFECTS OF TOTAL ASTRAGALOSIDES ON PROLIFERATION AND APOPTOSIS OF HUMAN LUNG CANCER CELL LINE A549[J]. Journal of Mudanjiang Medical College, 2008, 29(3): 16-18 |
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| Authors: | Niu Yan etal |
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| Affiliation: | Niu Yan etal (Tianli Medicine Limited Company of Mudanjaing,Mudanjaing Heilongjiang 157000) |
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| Abstract: | Objective:To observe the effect of Total Astragalosides (TA) on proliferation of human lung cancer cell line A549.Method:Using cell culture,MTT assay,DNA agarose gel electrophoresis and flow cytometry to observe the effect of TA on proliferation of human lung cancer cell line A549.Result:TA suppressed the growth of A549 cells markedly and induced apoptosis in a dose and time dependent manner.The treated cells exhibited obvious apoptosis.The characteristic ladder-like DNA fragment appeared in the cells.Concl... |
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| Keywords: | TA (Total astragalosides) human lung cancer cell line A549 proliferation apoptosis |
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