| 填充柱超临界流体色谱法测定枳实中辛弗林的含量 |
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| 引用本文： | 陆晓峰,王如健,彭国平,邓云天.填充柱超临界流体色谱法测定枳实中辛弗林的含量[J].辽宁中医杂志,2006,33(2):222-223. |
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| 作者姓名： | 陆晓峰 王如健 彭国平 邓云天 |
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| 作者单位： | 南京中医药大学植物药研究与新药开发中心,江苏,南京,210029 |
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| 摘 要： | 目的:建立填充柱超临界流体色谱法测定枳实中辛弗林含量的方法。方法:色谱柱:Berger PYR(乙基吡啶柱,250mm×4.6mm,6μm),流动相:超临界CO2-0.2%异丙胺甲醇(77∶23),压力:100bar,温度:35℃,流速:2.0mL/m in,检测波长:225nm。结论:辛弗林在0.71~11.36μg范围内呈良好的线性关系,r=0.9998;平均加样回收率为99.57%,RSD=1.69%。结论:该方法简便、快速、重复性好、结果准确,且流动相不需加入表面活性剂,辛弗林分离度高,可作为枳实中辛弗林的含量测定方法。
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| 关 键 词： | 超临界流体色谱 填充柱 枳实 辛弗林 |
| 文章编号： | 1000-1719(2006)02-0222-02 |
| 收稿时间： | 2005-09-02 |
| 修稿时间： | 2005年9月2日 |
Determination of the Content of Synephrine in Fructus Aurantii Immaturus by Packed-Column Supercritical Fluid Chromatography |
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| LU Xiao-feng,WANG Ru-jian,PENG Guo-ping,DENG Yun-tian.Determination of the Content of Synephrine in Fructus Aurantii Immaturus by Packed-Column Supercritical Fluid Chromatography[J].Liaoning Journal of Traditional Chinese Medicine,2006,33(2):222-223. |
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| Authors: | LU Xiao-feng WANG Ru-jian PENG Guo-ping DENG Yun-tian |
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| Abstract: | Objective: Methods for determination of the content of synephrine in Fructus Aurantii Immaturus by packed-column supercritical fluid chromatography was established.Methods: Column:Berger PYR(Ethyl pyridine,250mm×4.6mm,6μm);Mobile phase:CO_(2)-methanol with 0.2% isopropylamine(77∶23),Pressure: 100bar,Temperature:35℃,Flow:2.0mL/min,Detection wavelength:225nm.Results:The Synephrine had well linearity in the range of 0.71μg～11.36μg,r=0.9998;the average callback rate was 99.57%,RSD=1.69%.Conclusion:This method is convenient、quick、good refrain and accurate result,and mobile phase without surfactanus can be used for the quality control of synephrine in Fructus Aurantii Immaturus. |
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| Keywords: | supercritical fluid chromatography packed-Column Fructus Aurantii Immaturus synephrine |
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