Axial heterogeneity of sodium-bicarbonate cotransport in proximal straight tubule of rabbit kidney |
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Authors: | Y Kondo E Frömter |
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Institution: | (1) Zentrum der Physiologie, Klinikum der Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7, D-6000 Frankfurt/M. 70, Federal Republic of Germany;(2) Max Planck-Institut für Biophysik, Kennedyallee 70, D-6000 Frankfurt/M. 70, Federal Republic of Germany |
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Abstract: | Intracellular microelectrodes were used to investigate rheogenic Na+(HCO
3
–
)
n
cotransport in different segments of isolated proximal straight tubule (PST) of rabbit kidney. In the first portion (S2 segment) the peritubular cell membrane potentialV
b averaged –46.0, SE±1.3 mV (n=20), while in the terminal portion (S3 segment) it averaged –68.3, SE±2.5 mV (n=10). This difference may reflect different modes of anion permeation across the peritubular cell membrane. In S2 segments, sudden 10 1 reduction of bath HCO
3
–
concentration caused a fast transient cell depolarization, V
b=–45.8, SE±1.2 mV (n=33) as expected from the presence of Na+(HCO
3
–
)
n
contransport. As the puncture site moved further distally, V
b declined and gradually changed its time course by superposition of a slower secondary depolarization. In this region the transient cell depolarization could be recuperated by inhibiting the peritubular K+ conductance with Ba2+ (1 mmol/l). In S3 segments, however, the HCO
3
–
-dependent transient cell depolarization was completely lost both in the absence and presence of Ba2+. In addition, sudden reduction of bath Na+ concentration did not acidify the cell, as it did in the S2 segment. The data indicate that the expression of Na+(HCO
3
–
)
n
cotransport in the peritubular cell membrane gradually diminishes towards the end of the S2 segment and is lost in the S3 segment. |
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Keywords: | Rabbit kidney Proximal straight tubule S2/S3 segment Sodium-bicarbonate cotransport |
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