聚合酶链反应检测乙型肝炎患者肾组织中的HBV DNA

应用巢式聚合酶链反应（ＰＣＲ）技术对１１例乙型肝炎患者石蜡包埋的肾、肝组织中的ＨＢＶＤＮＡ进行了检测，并与其免疫组化及原位杂交结果作了比较。二组引物检测的结果表明：在肝组织中均有ＨＢＶ的感染，其中５例存有ＨＢＶ的复制；倚组织中ＨＢＶＤＮＡ的检出率为８／１１（７２．７％），未检测出有ＨＢＶ的复制，包括２例有膜性肾炎和膜增生性肾炎病变的病例。ＨＢＶ可以感染肾组织但并不在肾组织中复制。这一实验结果较为支持乙型肝炎患者肾脏出现的病变可能系沉积于肾小球内的免疫复合物介导损伤的结果。

Detection of HBV DNA in kidney by PCR

ested polymerase chain reaction (PCR)in two groups of primer was performed in formalin fixed, paraffin embedded kidney and liver tissues from 11 autopsjes of HBV infected patients for HBV DNA detection. DNA sequence representing HBV infection was detected in all the 11 liver tissue specimens(100%)and in 8 kidney tissue specimens(72.7%).DNA sequence representing HBV replication was de- tected in only 5 liver tissue specimens. The PC R findings corresponded with those obtained in immunohistochemistry studies and in situ hybridization,suggesting that HBV can infect the kidney but does not replicate in this organ and that the kidney pathology in HBV infected patients may be the result of immuno intermediate injury from immunocomplex deposited in glomeruli.