| 人类染色体8q24.1带36个单拷贝的克隆与筛选 |
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| 引用本文： | 黎伶俐,夏家辉,李麓芸,邓汉湘,何小轩,黄蕾,阮庆国,付俊江.人类染色体8q24.1带36个单拷贝的克隆与筛选[J].中国现代医学杂志,1994(2). |
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| 作者姓名： | 黎伶俐 夏家辉 李麓芸 邓汉湘 何小轩 黄蕾 阮庆国 付俊江 |
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| 作者单位： | 湖南医科大学医学遗传学国家重点实验室 410078
(黎伶俐，夏家辉，李麓芸，邓汉湘，何小轩，黄蕾，阮庆国)，湖南医科大学医学遗传学国家重点实验室 410078(付俊江) |
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| 摘 要： | 显微切割人类染色体8q24.1带DNA的初级PCR产物与pUC19质粒载体进行体外连接,转化大肠杆菌DH5a,获得了329个白色克隆。已分析的53个克隆中,有36个单拷贝,这些单拷贝片段长度介于105bp～960bp间,平均350bp,它们与正常人基因组DNA杂交显示各自特异性,可作为8q24.1带的特异性遗传标记。
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| 关 键 词： | 8q24.1带 显微切割 微克隆 单拷贝 |
CLONING AND SEREENING OF 36 SINGLE-COPY SEQUENCES FROM THE HUNAN CHROMOSOME BAND 8q24.1 |
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| Li Lingli et al
The State Key Laboratory of Medical Genetics,Hunan Medical University,Changsha, P.R.China..CLONING AND SEREENING OF 36 SINGLE-COPY SEQUENCES FROM THE HUNAN CHROMOSOME BAND 8q24.1[J].China Journal of Modern Medicine,1994(2). |
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| Authors: | Li Lingli
The State Key Laboratory of Medical Genetics Hunan Medical University Changsha PRChina |
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| Institution: | Li Lingli et al
The State Key Laboratory of Medical Genetics,Hunan Medical University,Changsha,410078 P.R.China. |
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| Abstract: | The primary PCR products obtained from the human chromosome band 8q24.1 by mi-crodissection techniques were digested and ligated in vitro into plasmid pUC 19. After transforming Escherichia coli DH5 a with above ligation mixture, we had got 329 transfor-mants. The insert fragments from 53 randomly chosen transformants were labelled to hybridize with normal human genomic DNA digested with EcoRI or Hind I and 36 of them were determined as single-copy sequences. Electrophoresis analysis revealed that these single-copy sequences had an average insert size of 350bp with a range of 105-96bp. Furthermore, because each of them shows special hybridization-band patterns with normal human genomic DNA, they can be regarded as the genetic landmarks for the human chromosome band 8q24. 1. |
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| Keywords: | Chromosome band 8q24 1 Microdissection and microclone Single-copy sequence |
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