Structural similarities of hog cholera virus with togaviruses |
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Authors: | P -J Enzmann F Weiland |
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Institution: | (1) Federal Research Institute for Animal Virus Diseases, Tübingen, Federal Republic of Germany |
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Abstract: | Summary Hog cholera virus grown in PK-15 cells was purified by centrifugation through a sucrose cushion followed by sucrose gradient centrifugation. Analysis of virus labeled externally with 3H]sodium borohydride on polyacrylamide gel electrophoresis revealed two glycoproteins, gp55 and gp46. A third structural polypeptide, p36, seems not to be glycosylated. The gp46 was also found in the virus-free supernatant of infected cells. It could be demonstrated by radioimmune precipitation of virus labeled with 35S]methionine that all three polypeptides are specific for hog cholera virions. Electron microscopically hog cholera virus appeared as a spherical particle with a diameter of 42±8 nm. The virus particles frequently displayed a fringe of projections with a length of about 6–8 nm. The similarities of hog cholera virus with Alphaviruses and Flaviviruses are discussed.With 5 Figures |
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