| 新生牛血清对大鼠脂肪来源细胞生长的影响 |
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| 引用本文： | 李萍,田方,宋琦,刘焕霞,李孟芳,魏丹. 新生牛血清对大鼠脂肪来源细胞生长的影响[J]. 遵义医学院学报, 2011, 34(4): 338-340 |
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| 作者姓名： | 李萍 田方 宋琦 刘焕霞 李孟芳 魏丹 |
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| 作者单位： | 遵义医学院附属口腔医院病例科,贵州遵义,563000 |
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| 基金项目： | 贵州省科技厅资助项目，遵义市科技局资助项目 |
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| 摘 要： | 目的探索新生牛血清对大鼠脂肪来源细胞生长状态的影响。方法用新生牛血清代替传统的胎牛血清对脂肪来源细胞进行体外培养,倒置相差显微镜下观察细胞形态,与胎牛血清培养条件下的脂肪来源细胞形态进行比较。结果新生牛血清培养条件下的大鼠脂肪来源细胞镜下呈短梭形或多角形,与胎牛血清培养的脂肪来源细胞形态相似,但细胞在P2～P3代即出现了衰老趋势。结论新生牛血清对体外培养的脂肪来源细胞的活力有负面影响,可能不适用于脂肪来源细胞的培养。
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| 关 键 词： | 脂肪来源细胞 新生牛血清 细胞培养 |
Effects of NBCS on the growth status of rat adipose tissue-derived cells |
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| LI Ping,TIAN Fang,SONG Qi,LIU Huanxia,LI Meng-fang,WEI Dan. Effects of NBCS on the growth status of rat adipose tissue-derived cells[J]. Acta Academiae Medicine Zunyi, 2011, 34(4): 338-340 |
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| Authors: | LI Ping TIAN Fang SONG Qi LIU Huanxia LI Meng-fang WEI Dan |
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| Affiliation: | LI Ping,TIAN Fang,SONG Qi,LIU Huanxia LI Meng-fang,WEI Dan(Affiliated Stomatological Hospital,Zuny i Medical College,Guizhou Zunyi 563000,China) |
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| Abstract: | Objective To investigate the effect of NBCS(new-born calf serum) on the growth status of rat adipose tissue-derived cells.Methods SD rat groin fat tissue was isolated by enzyme digestion.Adipose tissue-derived cells were cultured with medium plused 10%NBCS instead of the traditionally plused 10% FBS(fetal bovine serum).Cell morphology was assessed.Results Rat adipose tissue-derived cells under culture conditions with NBCS were observed by inverted phase-contrast microscope with short spindle and multi-angul... |
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| Keywords: | adipose tissue-derived cell NBCS cell culture |
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