| 红花多糖对人肝癌SMMC-7721细胞增殖的抑制作用 |
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| 引用本文： | 梁颖,张晓莉,陶冀,王亚贤. 红花多糖对人肝癌SMMC-7721细胞增殖的抑制作用[J]. 中医药学报, 2011, 39(5): 32-35 |
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| 作者姓名： | 梁颖 张晓莉 陶冀 王亚贤 |
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| 作者单位： | 黑龙江中医药大学,黑龙江哈尔滨,150040 |
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| 基金项目： | 黑龙江省自然科学基金项目(编号:D201050); 黑龙江省教育厅项目(编号:11551520) |
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| 摘 要： | 目的:观察红花多糖(Safflower polysaccharide,SPS)对SMMC-7721肝癌细胞增殖的抑制作用。方法:不同浓度SPS处理体外培养的SMMC-7721细胞株,采用MTT法测定SPS对SMMC-7721细胞增殖的影响;倒置显微镜观察细胞形态学变化;吖啶橙(AO)染色荧光显微镜观测以及Annexin-V/PI双染流式细胞仪检测各组细胞凋亡情况。结果:MTT法检测结果显示SPS对人肝癌SMMC-7721细胞体外增殖具有明显的抑制作用,该抑制作用呈明显的时间和剂量依赖性(P<0.05或P<0.01)。光镜下可见SPS组贴壁细胞数明显减少,细胞变小、变圆,折光性差,贴壁不牢,悬浮细胞增多。AO染色荧光显微镜下观察,24h部分细胞出现胞质浓缩,体积缩小,核固缩,染色增强,荧光更为明亮,形成致密浓缩的绿色荧光等;48h和72h部分细胞可见细胞核固缩为新月状。流式细胞术结果显示细胞凋亡率随药物浓度增加亦呈增长趋势。结论:SPS能显著抑制人肝癌细胞SMMC-7721生长且呈明显的量效和时效关系。SPS可诱导肝癌SMMC-7721细胞凋亡,具有一定的剂量依赖性。
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| 关 键 词： | SMMC-7721细胞 红花多糖 细胞增殖 细胞凋亡 |
The Inhibitory Effect of Safflower Polysaccharide on the Cell Proliferation of SMMC -7721 of Human Liver Cancer |
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| LIANG ying,ZHANG Xiao-li,TAO Ji,WANG Ya-xian. The Inhibitory Effect of Safflower Polysaccharide on the Cell Proliferation of SMMC -7721 of Human Liver Cancer[J]. Acta Chinese Medicine and Pharmacology, 2011, 39(5): 32-35 |
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| Authors: | LIANG ying ZHANG Xiao-li TAO Ji WANG Ya-xian |
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| Affiliation: | LIANG ying,ZHANG Xiao-li,TAO Ji,WANG Ya-xian(Heilongjiang University of Chinese Medicine,Harbin 150040,China) |
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| Abstract: | Objective:To observe the inhibitory effect of Safflower polysaccharide(SPS) on the cell proliferation of human liver cancer cell line SMMC-7721.Methods:SMMC-7721 cells were treated with different concentrations of SPS.The effect of SPS on the rates of cell proliferation was detected with MTT assay.Morphology changes were observed with inverted microscope.The effect of SPS on apoptosis was detected by FCM,SMMC-7721 cells were double-stained by Annexin-V/PI,and by fluorescence microscope,SMMC-7721 cells were ... |
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| Keywords: | SMMC-7721 cells Safflower polysaccharide(SPS) Cell proliferation Cell apoptosis |
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