| 参附注射液对大鼠心室肌细胞L型钙通道的影响 |
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| 引用本文： | 吴晓丹,孟红旭,张林,白晶,杨勇.参附注射液对大鼠心室肌细胞L型钙通道的影响[J].中医药信息,2011,28(6):55-58. |
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| 作者姓名： | 吴晓丹 孟红旭 张林 白晶 杨勇 |
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| 作者单位： | 1. 北京中医药大学,北京,100029
2. 中国中医科学院西苑医院,北京,100091 |
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| 基金项目： | 教育部中央高校基金项目，教育部留学回国人员科研启动基金项目 |
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| 摘 要： | 目的：观察参附注射液对大鼠心室肌细胞L型钙通道的作用。方法：采用急性酶解法获得单个心肌细胞,全细胞膜片钳技术记录钙电流。结果：参附注射液浓度依赖性阻断L型钙通道电流,其IC50为原液2.5%稀释。参附注射液（0.3%,3%）能够上移I-U曲线,但不改变峰电位和反转电位;3%参附注射液对L型钙通道激活曲线失活曲线无明显影响,但可使通道电流从失活中恢复的时间明显延长,时间常数由给药前的82ms增至给药后的139ms。结论：参附注射液对心肌细胞L型钙通道电流有抑制作用。
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| 关 键 词： | 参附注射液 心肌细胞 L型钙通道 膜片钳 |
The Effect of Shenfu Injection on L - type Calcium Current of Ventricular Myocytes in Rats |
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| WU Xiao-dan,MENG Hong-Xu,ZHANG Lin,BAI Jing,YANG Yong.The Effect of Shenfu Injection on L - type Calcium Current of Ventricular Myocytes in Rats[J].Information on Traditional Chinese Medicine,2011,28(6):55-58. |
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| Authors: | WU Xiao-dan MENG Hong-Xu ZHANG Lin BAI Jing YANG Yong |
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| Institution: | WU Xiao-dan2,MENG Hong-Xu2,ZHANG Lin1,BAI Jing1,YANG Yong1(1.Beijing University of Chinese Medicine,Beijing 100029,China,2.Xiyuan Hospital of China Academy of Chinese Medical Sciences,Beijing 100091,China) |
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| Abstract: | Objective: To study the effects of Shenfu injection(SF)on L-type calcium currents of ventricular myocytes in rats.Methods: The whole cell patch clamp technique was used to measure the L-type calcium currents in single myocytes dissociated by acute enzymatic dissociation method.Results: SF could block the L-type calcium currents in a concentration-dependent manner,IC50=2.5%;SF(0.3%,3%)made the I-V curves upward and did not change the reverse potential.SF in the concentration of 3% had no effect on the steady... |
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| Keywords: | Shenfu injection Ventricular myocytes L-type Calcium Currents Patch Clamp |
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