六味地黄汤相关苷类单体对小鼠脾淋巴细胞增殖作用的研究

目的：研究六味地黄汤中芍药苷、薯蓣皂苷元、马钱苷、莫诺苷、桃叶珊瑚苷对小鼠T、B淋巴细胞增殖的影响。方法：制备脾淋巴细胞,MTT比色法检测不同浓度5种苷类单体单独作用及其与丝裂原（ConA、LPS）共同作用时对小鼠T、B淋巴细胞增殖作用的影响。结果：10-5M芍药苷能显著促进淋巴细胞增殖,对LPS诱导的B淋巴细胞增殖有明显的协同作用,10-7～10-6M芍药苷对ConA诱导的T淋巴细胞增殖有明显协同作用,与对照组比较有显著性差异（P〈0.01,P〈0.05）;薯蓣皂苷元有一定促进淋巴细胞增殖作用,但与对照组比较无显著性差异;10-7～10-5M马钱苷能显著促进淋巴细胞增殖,10-7M马钱苷对LPS诱导的B淋巴细胞增殖有显著协同作用,随剂量增加,增殖作用反而降低;10-7～10-5M莫诺苷能显著促进淋巴细胞增殖,对LPS诱导的B淋巴细胞增殖有明显的协同作用,对ConA诱导的T淋巴细胞增殖有明显协同作用,与对照组比较有显著性差异（P〈0.01）。10-7～10-5M桃叶珊瑚苷基本无促进淋巴细胞增殖作用1,0-5M桃叶珊瑚苷对LPS诱导的B淋巴细胞增殖有明显的协同作用,10-7～10-5M桃叶珊瑚苷对ConA诱导的T淋巴细胞增殖有明显协同作用,与对照组比较有显著性差异（P〈0.01,P〈0.05）。结论：六味地黄汤中5种苷类单体对T、B淋巴细胞均有不同程度增殖作用,莫诺苷作用最明显,马钱苷低剂量促进增殖,高剂量反而抑制。

The Related Glycoside Monomer of Liuwei Dihuang Decoction and Proliferation of Splenic Lymphocytes in Mice

Objective: The effects of five compounds(paeoniflorin,diosgenin,loganin,morroniside and aucubin) in Liuwei Dihuang decoction on proliferative activities of mice splenic lymphocytes are investigated.Method: Paeoniflorin,diosgenin,loganin,morroniside and aucubin with or without concanavalin A(ConA) and lipopolysaccharide(LPS) were added into splenic lymphocyte.Effects of five compounds on the proliferative activities of T/B lymphocytes were measured by MTT colorimetric method.Results: Proliferative activities...