玻璃苣醇提取物对实验性哮喘豚鼠平喘作用机制的研究

目的：观察玻璃苣醇提取物对实验性哮喘豚鼠平喘作用机制的实验研究。方法：将48只健康SPF级豚鼠随机分为空白组,模型组,地塞米松组,玻璃苣醇提取物高、中、低剂量组,灌胃给药,每日1次,连续7天,同时每天均于给药后30min,以组织胺激发诱喘。取肺灌洗液测定其一氧化氮（NO）含量,嗜酸性粒细胞（EOS）数目,干扰素-γ（IFN-γ）,白细胞介素-4（IL-4）含量并计算其比值。结果：玻璃苣醇提取物使豚鼠肺灌洗液中EOS数目下降,NO浓度下降,IFN-γ含量升高,IL-4含量下降,比值升高。结论：玻璃苣醇提取物降低EOS在肺部的聚集,促进哮喘大鼠体内干扰素IFN-γ分泌和抑制IL-4表达,从而延长哮喘的潜伏期和降低支气管高反应性,达到治疗目的。

Antiasthmatic Mechanism of Borago Ethanol Extract on Guinea Pigs with Experimental Asthma

To investigate the antiasthmatic mechanism of Borago alcohol extract on guinea pigs.Methods： Forty-eight healthy SPF-class guinea pigs were randomly divided into blank group,model group and dexamethasone group.Borago alcohol extracts were administered orally once a day for seven days at high,medium and low doses.Thirty minutes after drug administration each day,histamine was given to the animals to induce asthma.NO,eosinophils,IFN-γ,and IL-4 content of lung lavage fluid were measured,and the ratio was calculated.Results： In the lung lavage fluid of guinea pig,the amount of EOS decreased,the NO concentrations were decreased by Borago alcohol extract,while the amount of IFN-γ was increased.The amount of IL-4 was decreased,while the ratio of it was increased.Conclusion： The ethanol extract of Borago can reduce aggregation of EOS in the lungs,promoting asthma rats interferon IFN-γ secretion and inhibition of IL-4 expression,thereby it can prolong the incubation period of asthma and reduce bronchial hyperresponsiveness for therapeutic purposes.