| 具有乙酰胆碱酯酶抑制活性的总生物碱提取物的筛选 |
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| 引用本文： | 杨中铎,赖东海,张东博,杨明俊.具有乙酰胆碱酯酶抑制活性的总生物碱提取物的筛选[J].中医药学报,2011,39(3):71-74. |
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| 作者姓名： | 杨中铎 赖东海 张东博 杨明俊 |
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| 作者单位： | 兰州理工大学生命科学与工程学院,甘肃兰州,730050 |
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| 基金项目： | 国家自然科学基金，兰州理工大学优秀青年教师培养计划资助项目 |
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| 摘 要： | 目的:从采自甘肃文县和舟曲的22种植物的总生物碱提取物中筛选具有乙酰胆碱酯酶抑制活性的提取物,为进一步寻找天然乙酰胆碱酯酶抑制剂单体成分奠定基础。方法:采用酸提碱沉法提取生物碱,用改良的Ellman比色法测定总生物碱的抗乙酰胆碱酯酶活性。结果:弯柱唐松草(Thalictrumuncinulatum Franch.)、阔叶十大功劳(Mahnia bealei(Fort.)Steam.)、蚬壳花椒(Zanthoxylum dissitumHemsl.)、刺柄南星(Arisaema aspeiatum N.E.Brown)、一把伞南星(Arisaema erunescens(Wall.)Schott)、猫儿屎(Decaisnea insignis(Griff)Hook.f.et thoms)、虎耳草(Saxifraga stolonifera curt.)和西固凤仙花(Impot-iens notolopha Maxim.)的总生物碱提取物具有明显的乙酰胆碱酯酶抑制活性,其IC50值分别为2.3μg.mL-1、2.9μg.mL-1、3.5μg.mL-1、11.0μg.mL-1、16.9μg.mL-1、22.7μg....
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| 关 键 词： | 乙酰胆碱酯酶 抑制剂 生物碱 活性评价 |
Screening of Total Alkaloidal Extracts from Plant for Acetylcholinesterase Inhibitory Activity |
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| YANG Zhong-duo,LAI Dong-hai,ZHANG Dong-bo,YANG Ming-jun.Screening of Total Alkaloidal Extracts from Plant for Acetylcholinesterase Inhibitory Activity[J].Acta Chinese Medicine and Pharmacology,2011,39(3):71-74. |
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| Authors: | YANG Zhong-duo LAI Dong-hai ZHANG Dong-bo YANG Ming-jun |
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| Institution: | YANG Zhong-duo,LAI Dong-hai,ZHANG Dong-bo,YANG Ming-jun(School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,China) |
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| Abstract: | Objective:To assess acetylcholinesterase(AChE) inhibitory activity of 22 species of plants total alkaloidal extracts which are collected from Wenxian and Zhouqu,Gansu Province for the further research for natural acetylcholinesterase inhibitors.Methods:The total alkaloids were extracted by acid and alkali aqueous solution treatment.The AChE inhibitory activity was determined by modified Ellman's colorimetric method in 96-well microplates.Results:The results showed that the total alkaloidal extracts from Tha... |
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| Keywords: | Acetylcholinesterase Inhibitor Alkaloid Activity evaluation |
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