| 白细胞介素4-绿脓杆菌外毒素冻干保护剂的筛选 |
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| 引用本文： | 胡志明,陈泽洪,孟晓静,李宏增,林来兴妹,王小宁. 白细胞介素4-绿脓杆菌外毒素冻干保护剂的筛选[J]. 南方医科大学学报, 2001, 0(Z1) |
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| 作者姓名： | 胡志明 陈泽洪 孟晓静 李宏增 林来兴妹 王小宁 |
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| 作者单位： | 第一军医大学分子免疫研究所，第一军医大学分子免疫研究所，第一军医大学分子免疫研究所，第一军医大学分子免疫研究所，第一军医大学分子免疫研究所，第一军医大学分子免疫研究所 广东 广州 510515，广东 广州 510515，广东 广州 510515，广东 广州 510515，广东 广州 510515 |
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| 摘 要： | 目的筛选白细胞介素4-绿脓杆菌外毒素最佳冻干保护剂配方。方法设计12组不同的保护剂配方,用U251细胞为靶细胞测定冻干品的活性,并对外观、溶解性进行比较。结果使用5％甘露醇+0.004％吐温80组保护剂,冻干品的活性与冻干前没有降低,冻干品外观、溶解性均优于其它配方。结论5％甘露醇0.004％吐温80组保护剂为最佳侯选保护剂配方。
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| 关 键 词： | 白细胞介素4-绿脓杆菌外毒素 保护剂 冻干 |
Screening of the protective agent for freeze-dried interleukin-4-pseudomonas |
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| HU Zhi-ming,CHEN Ze-hong,MENG Xiao-jing,LI Hong-zeng,LINLAI Xing-mei,WANG Xiao-ning Institute of Molecular Immunology,First Military Medical University,Guangzhou ,China. Screening of the protective agent for freeze-dried interleukin-4-pseudomonas[J]. Journal of Southern Medical University, 2001, 0(Z1) |
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| Authors: | HU Zhi-ming CHEN Ze-hong MENG Xiao-jing LI Hong-zeng LINLAI Xing-mei WANG Xiao-ning Institute of Molecular Immunology First Military Medical University Guangzhou China |
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| Affiliation: | HU Zhi-ming,CHEN Ze-hong,MENG Xiao-jing,LI Hong-zeng,LINLAI Xing-mei,WANG Xiao-ning Institute of Molecular Immunology,First Military Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To screen the protective agent for freeze-dried interleukin-4-pseudomonas. Methods Twelve kinds of protective agents were designed and U251 cells were used as target cells to test the bioactivity of freeze-dried inter-leukin-4-pseudomonas. Results The bioactivity of interleukin-4-pseudomonas treated with the protective agent group 4 (5% mannitol plus 0.004% tween 80) did not decrease as compared with that of control group. Conclusion 5% mannitol plus 0.004% tween 80 is the optimum candidate protective agent for freeze-dried interleukin-4-pseudomonas. |
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| Keywords: | interleukin-4-pseudomonas protective agent freeze-dry |
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