Nec-1对铝作用下神经细胞活力及凋亡、自吞噬基因的影响

目的]研究坏死性抑制剂Necrostatin-1（Nec-1）对铝（Al）作用下的神经细胞活力及凋亡、自吞噬基因的影响。方法]体外原代培养小鼠神经细胞，通过2mmol／LAl3＋作用于神经细胞制造铝损伤模型，加入不同剂量的Nec-1，用细胞活力检测（CCK-8）和逆转录．聚合酶链反应（RT．PCR）的方法观察Nec-1对染铝神经细胞的作用。结果]细胞活力检测：以2mmol／LAl3＋染毒组作为对照，30μmol／LNec-1组的细胞活力与对照组比较，差别有统计学意义（P〈0．05），60、90μmol／LNec-1组与对照组比较，差异均有统计学意义（P〈0．01）.RT-PCR结果：2mmol／LAl3＋染毒组caspase-3基因的表达为0．98±0．120，而Nec-160μmol／L组和Nec-190μmol／L组caspase-3的表达分别为0．50±0．077和0．44±0．050，同对照组相比，两组caspase-3的表达均下降（P〈0．01）。2mmol/LAl3＋染毒组的LC3-Ⅱ基因的表达为1,82±0．047，而60μmol／LNec-1组和90μmol／LNec-1组的LC3．口表达分别为1．00±0．022和0．97±0．035，同对照组相比，两组LC3-Ⅱ的表达均呈下降（P〈0．01）。结论]Nec-1可使铝作用下的神经细胞活力上升，并使神经细胞的自吞噬和凋亡基因表达下降。

Effect of Nec-1 on Cell Viability and Genes of Apoptosis and Autophagy in the Aluminum-induced Nerve Cells

Objective ] To explore the effect of Nee-1 on cell viability and genes of apoptosis and autophagy of the aluminun-induced nerve cells. Methods ] We conducted the study by means of cell culture in vitro, producing aluminum injury model by Al3＋（ 2 mmol/L ）, then intervented cell death by Necrostatin- 1 （ Nee-1 ）, and investigated the Nee-1 ＇s role toward the Al- induced nerve cells using CCK-8 and RT-PCR methods. Results ] Cell viability of Nee-1 （ 30 μmol/L ）group had statistically difference （ P 〈 0.05 ）, whereas of the Nec-1 （ 60 μmol/L ） and Nee-1 （ 90 μmol/L ） groups had very significant difference （ P 〈 0.01 ）as compared with AP（ 2 mmol/L ）exposure group （ control ）.The RT-PCR demonstrated that the expression of caspase-3 gene in Al3＋ （ 2 mmol/L ） group （ control ） was 0.98 ± 0.120, but that of Nee- 1 （ 60 μmol/L ） and Nee- 1 （ 90 μmol/L ） groups were 0.50 ＋ 01077 and 0.44 ＋ 0.050 respectively. Compared with the control group, the expression of caspase-3 gene of the latter two groups decreased with statistical significance（ P 〈 0.01 ）. The expression of LC3-Ⅱgene of Al3＋（ 2 mmol/L ）group（ control ）was 1.82 ± 0.047, but that of Nee-1 （60 μmol/L ）and Nee-1 （90 μmol/L ）groups were 1.00 ± 0.022 and 0.97 ± 0.035 respectively. Compared with the control group, the expression of LC3-Ⅱ gene of the latter two groups also decreased with statistical significance（ P 〈 0.01 ）. Conclusion ] In summary, Nee-1 can increase cell viability of Al-induced nerve cells, and decrease the expression of autophagy and apoptosis genes.