A genetic analysis of glucoamylase activity in the diastatic yeast Saccharomyces cerevisiae NCYC 625 |
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| Authors: | Daksha Patel Ivor H Evans E Alan Bevan |
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| Institution: | (1) Howard Hughes Medical Institute Research Laboratory, Department of Molecular Genetics & Cell Biology, University of Chicago, 920E58th Street, 6037 Chicago, Illinois, USA;(2) School of Biological Sciences and Environmental Health, Thames Polytechnic, Wellington Street, SE18 6PF London, UK;(3) School of Biological Sciences, Queen Mary College, Mile End Road, E1 4NS London, UK |
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| Abstract: | Summary The wild diastatic yeast Saccharomyces cerevisiae NCYC 625 has been shown to be homozygous for the glucoamylase-specifying gene STA2. spoII-1-mapping has positioned STA2 on chromosome II. Expression of STA2 is suppressed in some but not all diploids capable of sporulation, and is also inhibited by unlinked nuclear suppressor genes (SGL) found in some S. cerevisiae tester strains. EMS-induced glucoamylase-negative mutants often contain STA2-suppressor mutations. Depending on the allelic status of GEP1, a nuclear gene which also appears able to antagonise SGL-mediated suppression, STA2 expression can be blocked in petite mutants. |
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| Keywords: | Glucoamylase Gene-mapping STA2 Mitochondria |
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