红景天苷类似物合成及微孔板定量法评估其抗氧化能力 |
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引用本文: | 郭益冰,邵健,郯成,孟颖,杨宇民.红景天苷类似物合成及微孔板定量法评估其抗氧化能力[J].中国交通医学杂志,2012,26(1). |
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作者姓名: | 郭益冰 邵健 郯成 孟颖 杨宇民 |
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作者单位: | 郭益冰 (南通大学附属医院外科综合实验室) ; 邵健 (江苏省神经再生重点实验室,江苏226001) ; 郯成 (江苏省神经再生重点实验室,江苏,226001) ; 孟颖 (江苏省神经再生重点实验室,江苏,226001) ; 杨宇民 (江苏省神经再生重点实验室,江苏,226001) ; |
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基金项目: | 国家自然科学基金资助项目,南通市社会发展计划,南通大学自然科学基金资助项目 |
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摘 要: | 目的:合成红景天苷类似物,并初步分析评价其抗氧化能力。方法:以O-乙酰基溴代葡萄糖、O-乙酰基溴代半乳糖为糖基供体,芳环不同取代的苯甲醇、苯乙醇为糖基配体通过Koenigs-Knorr法制备红景天苷类似物,并采用微孔板定量法分析测定其对2,2-二苯基苦基苯肼自由基(DPPH.)的清除能力。结果:合成了10个类似物糖苷,确定了其在清除DPPH.反应达到50%时的IC50(μmol/L)。结论:所合成类似物3-(3,4,5-三羟基苯基)丙基-β-D-葡萄糖苷和3-(3,4,5-三羟基苯基)丙基-β-D-半乳糖苷具有较好的抗氧化能力,其IC50分别为36.7μmol/L和39.7μmol/L,优于阳性对照维生素CIC50(54.6μmol/L)。
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关 键 词: | 红景天苷 微孔板定量测定 类似物 2 2-二苯基苦基苯肼自由基 |
Synthesis and Evaluation of Antioxidative Capability of Salidroside Analogues by Microassay |
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Abstract: | Objective: To synthesize salidroside analogues and evaluate its antioxidative capability tentatively.Method: The synthesis was conducted through Koenigs-Knorr method,O-acetobrome-sugar(glucose and galactose) as glycon donor and different substituted benzyl alcohol and phenylethyl alcohol as aglycone,and microplate quantification(MQ) of 2,2-diphenyl-1-picrylhydrazyl(DPPH·) was used to evaluate its scavenging capability.Results: Ten salidroside analogues were synthesised,the IC50(μmol/L) of which were afforded.Conclusion: Compounds 3-(3,4,5-Trihydroxyphenyl) propyl β-D-glucopyranoside and 3-(3,4,5-Trihydroxyphenyl) propyl β-D-galactopyranoside displayed good antioxidant capacity,IC50 being 36.7 μmol/L and 39.7 μmol/L,respectively,superior to VCIC50(54.6 μmol/L) in the positive control. |
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Keywords: | salidroside Microplate quantification assay Analogues 2 2-diphenyl-1-picrylhydrazyl |
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