|
|||||
|
|
| CD45在克罗恩病患者血清中的表达 | |
| 引用本文： | 康亮,杨祖立,王磊,黄美近,刘炜,黎明涛,汪建平.CD45在克罗恩病患者血清中的表达[J].南方医科大学学报,2009,29(2):259. |
| 作者姓名： | 康亮 杨祖立 王磊 黄美近 刘炜 黎明涛 汪建平 |
| 作者单位： | 1. 中山大学附属第六医院/胃肠肛门医院结直肠外科,广东,广州,510655 2. 中山大学附属第六医院/胃肠肛门医院胃肠外科,广东,广州,510655 3. 中山大学基础医学院蛋白质组学实验室,广东,广州,510080 |
| 基金项目： | 广东省自然科学基金，广东省自然学基金博士启动项目 |
| 摘 要： | 目的 应用蛋白质组学方法在患者血清中寻找与克罗恩病相关的蛋白质.方法 采取克罗恩病患者以及正常成人血清蛋白样本各4例,用不同的CyDye荧光染料标记后进行胶内差异双向凝胶电泳(2-D DIGE),并对获得的图谱进行分析及对差异蛋白质进行基质辅助激光解吸离子化飞行时间质谱鉴定.结果 通过2-D DIGE分析,发现克罗恩病患者中存在29个表达异常蛋白质点,其中胶号为973的蛋白质点,在克罗恩病患者患者血清中比正常成人组表达升高2.55倍(P<0.05),该蛋白点经质谱鉴定为CD45.结论 CD45在克罗恩病患者血清的高表达提示在克罗恩病的病程中免疫系统失衡可能起着一定作用. |
| 关 键 词： | 克罗恩病 双向胶内差异凝胶电泳 基质辅助激光解吸离子 质谱 |
Expression of CD45 in the serum of patients with Crohn's disease |
|
| KANG Liang,YANG Zu-li,WANG Lei,HUANG Mei-jin,LIU Wei,LI Ming-tao,WANG Jian-ping.Expression of CD45 in the serum of patients with Crohn's disease[J].Journal of Southern Medical University,2009,29(2):259. | |
| Authors: | KANG Liang YANG Zu-li WANG Lei HUANG Mei-jin LIU Wei LI Ming-tao WANG Jian-ping |
| Institution: | KANG Liang1,YANG Zu-li2,WANG Lei1,HUANG Mei-jin1,LIU Wei3,LI Ming-tao3,WANG Jian-ping1 1Department of Colorectal Surgery,2Department of Gastroenterology Surgery,Sixth Affiliated Hospital of Sun Yat-sen University,Guangzhou 510655,China,2Laboratory of Proteomics,School of Preclinical Medicine,Sun Yat-sen University,Guangzhou 510080 |
| Abstract: | Objective To search for differentially expressed proteins in the serum of patients with Crohn's disease. Methods Serum protein samples obtained from 4 patients with Crohn's disease and 4 normal adults were cross-labeled with different CyDyes and underwent two-dimensional differential in-gel electrophoresis (2-D DIGE) and imaging analysis. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to identify the differentially expressed proteins. Results 2-D DIGE re... |
| Keywords: | CD45 |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《南方医科大学学报》浏览原始摘要信息 | |
| 点击此处可从《南方医科大学学报》下载免费的PDF全文 | |