Clonal osteogenic cell lines express myogenic and adipocytic developmental potential |
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Authors: | Akira Yamaguchi Arnold J Kahn |
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Institution: | (1) Department of Oral Pathology, School of Dentistry, Showa University, Tokyo, Japan;(2) Department of Growth and Development, School of Dentistry, University of California, San Francisco, 521 Parnassus Avenue, 94122 San Francisco, California, USA |
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Abstract: | Summary Clonal osteoblastic cell lines were isolated from neonatal rat calvariae and characterized with regard to a number of features
associated with authentic osteoblasts. These included elevated alkaline phosphatase activity (relative to fibroblasts), PTH
and PGE2-stimulated increases in cAMP, the predominant synthesis of type 1 collagen, and the production of a mineralized matrixin vitro. By these criteria, five clones with osteoblast-like phenotypes were identified (ROB-C8a, C11, C20, C23, and C26) which varied
somewhat in shape, levels of alkaline phosphatase activity, and in responsiveness to PTH and PGE2, C11, C20, and C23 responded to both effector substances, whereas C8a only responded to PTH and C26 only responded strongly
to PGE2. Upon further examination, two of the clones (C23 and C26) were also found to exhibit significant muscle myotube formation
after reaching confluence, and three of the clones (C8a, C11, and C26) showed marked adipocyte differentiation after treatment
with dexamethasone. Overall, these data add further supporting documentation to (1) the suspected ontogenetic relationships
of osteoblasts to other connective tissue cells, and (2) the concept that osteoblastic cells associated with neonatal rat
calvariae are in various stable stages of differentiation and developmental commitment. |
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Keywords: | Osteoblastic cell lines cAMP PTH PGE2 ALP Mitotubes Adipocytes |
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