| 三氧化二砷对MRL/lpr自发性狼疮小鼠脾脏单个核细胞DNMT1及CD_(11a)表达的影响 |
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| 引用本文： | 王红,孙莉,陈培荣,周艳,朱小春. 三氧化二砷对MRL/lpr自发性狼疮小鼠脾脏单个核细胞DNMT1及CD_(11a)表达的影响[J]. 浙江医学, 2008, 30(11) |
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| 作者姓名： | 王红 孙莉 陈培荣 周艳 朱小春 |
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| 作者单位： | 1. 温州医学院附属第二医院肾病风湿免疫科,325027
2. 学院附属第一医院风湿免疫科,325027 |
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| 基金项目： | 浙江省中医药科技研究计划 |
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| 摘 要： | 目的探讨三氧化二砷（ATO）对MRL/lpr自发性狼疮小鼠脾脏单个核细胞（SMC）中DNA甲基转移酶1（DNMT1）和黏附分子淋巴细胞功能相关抗原-1（LFA-1）的亚基CD11a表达的影响及其可能作用机制。方法分离小鼠SMC,采用20g/ml植物血凝素和1000IU/ml白介素-2体外诱导培养SMC 48h后,随机分为空白组、ATO组和5-氮胞苷（5-Az）组,继续培养24h,采用RT-PCR法检测两组小鼠SMC中DNMT1及CD11a基因转录水平的表达情况。结果MRL/lpr小鼠ATO组中DNMT1-mRNA的灰度比值较5-Az组增高（P〈0.05）,MRL/lpr小鼠ATO组中CD11a-mRNA的灰度比值较5-Az组降低（P〈0.05）。结论ATO治疗系统性红斑狼疮的作用机制可能与其能逆转低甲基化状态有关。
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| 关 键 词： | 三氧化二砷 5-氮胞苷 系统性红斑狼疮 DNA甲基转移酶1 |
Effects of arsenic trioxide on the expression of DNA methyltransferase 1 and CD(11a) genes in splenic mononuclear cells of spontaneous lupus nephritis MRL/lpr mice |
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| WANG Hong,SUN Li,CHEN Peirong,et al.. Effects of arsenic trioxide on the expression of DNA methyltransferase 1 and CD(11a) genes in splenic mononuclear cells of spontaneous lupus nephritis MRL/lpr mice[J]. Zhejiang Medical Journal, 2008, 30(11) |
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| Authors: | WANG Hong SUN Li CHEN Peirong et al. |
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| Affiliation: | WANG Hong,SUN Li,CHEN Peirong,et al. Department of Nephrology,the Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325027,China |
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| Abstract: | Objective Toinvestigate the effect of arsenic trioxide(ATO) on the expression of DNA methyltransferase1 (DNMT1)mRNA and CD11a mRNA in splenic mononuclear cells (SMNC) of spontaneous lupus nephritis MRL/lpr mice. Methods Isolated SMNC of MRL/lpr and C57BL mice were induced by PHA 20g/ml + IL-2 1000IU/ml for 48 h, and the cultured cells were randomly divided into control group, ATO group, 5-AZ group. Each group was incubated in vitro for 24h and RNA was extracted. The expression of DNMT1 and CD11a was determi... |
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| Keywords: | CD11a |
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