cagA vacA alelles and babA2 genotypes of Helicobacter pylori associated with gastric disease in Brazilian adult patients |
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| Authors: | Gatti Luciano Lobo Fagundes e Souza Ellen Kris Leite Kátia Ramos Bastos Eduardo Lemos de Souza Vicentini Luciano Roberto Silva Luiz Carlos da Smith Marília de Arruda Cardoso Payão Spencer Luiz Marques |
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| Institution: | 1. University of Pittsburgh, Department of Immunology, Pittsburgh, PA, USA;2. Children’s Hospital of Pittsburgh, RK Mellon Institute, Department of Pediatrics, Pittsburgh, PA, USA;3. Cancer Immunology and Immunotherapy Program, UPMC Hillman Cancer Center, Pittsburgh, PA, USA |
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| Abstract: | Helicobacter pylori is an important human pathogen that causes chronic gastritis and is associated with development of peptic ulcer disease and gastric malignancies. The vacuolating cytotoxin (vacA), cagA gene, and babA2 gene are important virulence factor involving gastric diseases. Eighty-nine Helicobacter pylori-positive gastric biopsies were analyzed by polymerase chain reaction and Southern blotting for H. pylori detection and genotyping with primer pairs from each virulence gene. Fifty-three strains (59%) were common vacA genotype s1/m1, and only 14 (16%) were s2/m2, 12% of strains was found to have multiple infection. The cagA presence was detected in 48% (43 strains) and babA2 gene was detected in 44% of our H. pylori strains. We observed high percentage of s1/m1 strains with chronic gastritis and peptic ulcer and a significant correlation between cagA presence with the s1 allele and babA2 gene with chronic gastritis. |
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