| CDKN2/p16基因存在状态与肺癌 |
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| 引用本文： | 叶玉坤,苏长青,汪栋,张志敏,张卫兵,单祥年.CDKN2/p16基因存在状态与肺癌[J].中华胸心血管外科杂志,2000,16(5):277-279. |
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| 作者姓名： | 叶玉坤 苏长青 汪栋 张志敏 张卫兵 单祥年 |
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| 作者单位： | 1. 南京,解放军八一医院全军肿瘤研究中心,210002
2. 南京师范大学生物系 |
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| 基金项目： | 国家“九五”重点科技项目!(攻关)计划96-90 6-01-18)，国家自然科学基金资助课题!(39670714) |
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| 摘 要： | 目的 研究CDKN2 /p16基因缺失和蛋白丢失在肺癌发展中的作用。方法 采用免疫组化和改良标本处理的PCR技术 ,对 89例肺癌病人手术标本CDKN2 /p16基因第 1、2外显子纯合缺失和P16蛋白表达丢失情况进行分析对比研究。结果 CDKN2 /p16基因第 1或 (和 )第 2外显子总缺失率为2 5 8% (2 3/ 89) ,P16蛋白丢失率为 47 2 % (4 2 / 89) ,基因的缺失和蛋白的丢失集中发生于非小细胞肺癌(NSCLC) ,并与转移和分期有关。结论 CDKN2 /p16基因的异常可能是NSCLC区别于小细胞肺癌(SCLC)的特有的分子事件 ,其在NSCLC的发生发展中起一定的负调控作用
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| 关 键 词： | 肺肿瘤 CDKN2/p16基因 免疫组织化学 聚合酶链反应 |
Study of CDKN2/p16 gene existence state in lung cancer |
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| YE Yukun,SU Changqing,WANG Dong,et al..Study of CDKN2/p16 gene existence state in lung cancer[J].Chinese Journal of Thoracic and Cardiovascular Surgery,2000,16(5):277-279. |
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| Authors: | YE Yukun SU Changqing WANG Dong |
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| Institution: | YE Yukun,SU Changqing,WANG Dong,et al. Cancer Center of PLA,Nanjing 81 Hospital,Nanjing 210002 |
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| Abstract: | Objective To study CDKN2/p16 gene homozygous deletion and p16 protein loss in the genesis and progression of lung cancer. Methods Improved multiplex PCR technique and immunohistochemistry were applied to detect deletion of CDKN2/p16 gene exon1 and exon2 and loss of p16 protein in the 89 cases of lung cancer. Results Total rate of exon1 or/and exon2 deletion was 25 8%(23/89), and p16 protein loss rate was 47 2%(42/89) in lung cancer. CDKN2/p16 gene deletion and protein losses occurred in NSCLC, and were related to metastasis and progressive stage. Conclusion Abnormality of CDKN2/p16 gene is a specific molecular event in NSCLC, and may play an important role in the progression of NSCLC. |
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| Keywords: | Lung neoplasms CDKN2/p16 gene Immunohistochemistry Polymerase chain reaction |
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