大黄素对内皮细胞一氧化氮合成的影响 |
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引用本文: | 潘浩,张邢炜,王宁夫,杨建敏,周亮,徐坚,叶显华,李佩璋.大黄素对内皮细胞一氧化氮合成的影响[J].中国现代医生,2011,49(16):101-103. |
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作者姓名: | 潘浩 张邢炜 王宁夫 杨建敏 周亮 徐坚 叶显华 李佩璋 |
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作者单位: | 1. 杭州市第一人民医院心内科,浙江杭州,310006 2. 杭州师范大学附属医院心内科,浙江杭州,310015 |
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基金项目: | 浙江省杭州市医学重点专科专病科研项目 |
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摘 要: | 目的观察大黄素对内皮细胞一氧化氮及一氧化氮合酶合成的影响。方法通过选用新生儿脐静脉,采用灌注消化法建立内皮细胞模型,分别取10.0mg/L、37.5mg/L、75.0mg/L大黄素作用于细胞模型,测定细胞培养液中NO、NOS含量。结果大黄素在75.0mg/L浓度时NO、NOS合成明显增加,与对照组比较差异有统计学意义(P〈0.05),且其合成是脉冲式的。结论大黄素可以通过调控NOS的合成,促进NO的生成,对内皮细胞具有促进其功能的作用。
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关 键 词: | 大黄素 内皮细胞 一氧化氮 一氧化氮合酶 |
Effects of Emodin on Synthesis of Nitric Oxide in Human Endothelial Cells |
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Authors: | PAN Hao ZHANG Xingwei WANG Ningfu YANG Jianmin ZHOU Liang XU Jian YE Xianhua LI Peizhang |
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Institution: | 1.Department of Cardiology,Hangzhou First People's Hospital, Hangzhou 310006,China;2.Department of Cardiology,The Affiliated Hospital of Hangzhou Normal University,Hangzhou 310015,China) |
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Abstract: | Objective To observe the changes of nitric oxide (NO) content and nitric oxide synthase (NOS) activity in human umbilical vein endothelial cells (HUVECs) by emodin. Methods The human umbilical vein endothelial ceils were cultured by digest method for 2-3 posterities. HUVECs were incubated with emodin (10.0mg/L,37.5mg/L,75.0mg/L) and analyzed by colorimetric method and spectrophotometric methods. Results The content of NO and the activity of NOS were significantly higher in 75.0mg/L emodin group than those of normal control group. Conclusion Emodin can increase the content of NO and the activity of NOS in the human umbilical vein endothelial cells which presents the protective effects of emodin. |
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Keywords: | Emodin Endothelial cell Nitric oxide Nitric oxide synthase |
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