环磷酰胺和嗜水气单胞菌对暗纹东方免疫抑制的研究

对暗纹东方分别注射 8、 0 8、 0 0 8mg/kg·bw的环磷酰胺 ,5d一次 ,连续 3次 ,并与一次性注射嗜水气单胞菌 (1× 1 0 5CFU/kg·bw )相比较 ,研究其对暗纹东方免疫功能的影响。结果显示 ,注射适当浓度的环磷酰胺和嗜水气单胞菌可明显减弱暗纹东方肝胰脏溶菌酶活性、脾脏NK细胞杀伤率、脾脏B淋巴细胞转化能力 ,显著降低白细胞吞噬指数和血清干扰素 (IFN α )含量 (P <0 0 5 )。选择 8mg/kg·bw环磷酰胺和 1× 1 0 5CFU/kg·bw的嗜水气单胞菌作为注射浓度可以抑制暗纹东方的免疫功能。相比之下 ,在试验范围内 ,环磷酰胺对暗纹东方免疫能力的抑制程度更大 ,范围更广 ,稳定性更好。

The Immuno-Suppressive Effects of Cyclophosphamide and Aeromonas Hydrophila on Fugu Obscurus

The influence of the intra-peritoneal injection of cyclophosphamide(Cy), 8, 0.8 and 0.08 mg/kg·bw every 5 days for 3 times, and the bacterial suspensions of Aeromonas hydrophila, 1×105 CFU/kg·bw, once, on the immune functions of Fugu obscurus was investigated in the present study. It was found that the lysozyme activity in liver, the cytotoxic activity of NK cells and the transforming ability of B cells in spleen, phagocytic index of leukocytes and the serum contents of IFN-α of Fuga obscurus were significantly decreased after injections of Cy and Ah at optimal concentrations (8 mg/kg·bw and 1×105CFU/kg·bw respetive), but the inhibitory effects on Fuga obscurus were more obvious with a broad range of actions and steady stability in case of injection with Cy than that of Ah.