Immunomagnetic T-lymphocyte depletion (ITLD) of rat bone marrow using OX-19 monoclonal antibody |
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| Authors: | A L Hoffman L Makowka D V Cramer X Cai A Pascualone B F Banner D G Sheahan P Rettinger T E Starzl |
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| Affiliation: | Department of Surgery, University of Pittsburgh School of Medicine, PA 15261. |
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| Abstract: | Graft versus host disease (GVHD) may be abrogated and host survival prolonged by in vitro depletion of T lymphocytes from bone marrow (BM) prior to allotransplantation. Using a mouse anti-rat pan T-lymphocyte monoclonal antibody (OX19) bound to monosized, magnetic, polymer beads, T lymphocytes were removed in vitro from normal bone marrow. The removal of the T lymphocytes was confirmed by flow cytometry. Injection of the T-lymphocyte-depleted bone marrow into fully allogeneic rats prevents the induction of GVHD and prolongs host survival. |
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