慢性束缚应激模型对小鼠社交行为的影响

目的 探讨慢性束缚模型对小鼠社交行为的影响，为应激抑郁模型的社会行为学评价方法提供数据支撑。方法 采用慢性束缚加孤养方法建立应激抑郁模型，模型组每日束缚4 h（10:00-14:00），连续进行35 d，单笼饲养。应用糖水偏爱测试确定模型成功，应用社会趋避实验评价应激抑郁模型对小鼠社交行为的影响。结果 糖水偏爱实验结果显示，慢性束缚组糖水偏爱指数明显下降，与正常组比较具有显著性差异（P<0.01）。社会趋避实验结果显示，正常组对刺激鼠笼的嗅闻时间及频次多于空鼠笼，并具有显著性差异（P<0.05，P<0.01）；慢性束缚组对刺激鼠笼和空鼠笼的嗅闻时间及频次相比，无显著性差异。慢性束缚组嗅闻刺激鼠笼时间及频次明显少于正常组，二者相比具有显著性差异（P<0.05，P<0.01）。慢性束缚组动物第一次嗅闻刺激鼠笼的潜伏期明显比正常组长，二者相比具有极显著性差异（P<0.01）。慢性束缚组对刺激鼠笼的偏好指数明显小于正常组，二者具有极显著性差异（P<0.01）。结论 慢性束缚应激小鼠社交回避的行为学表现，为应激所致抑郁模型社会行为学评价体系的建立提供数据支撑。

Effect of chronic restraint stress on sociability in mice

Objective To investigate the effect of chronic restraint stress on sociability in mice. Method Chronic restraint stress was applied to establish stress depression model. The mice were individually housing and restrained for 4 h per day in a 50 mL polypropylene conical tube with ventilation holes. This daily restraint was repeated for 35 consecutive days. The sucrose preference test was applied to evaluate the model. The social approach-avoidance test was used to assess the effect of chronic restraint stress on sociability. Results The sucrose intake was significantly reduced in chronic restraint stress model compared with the control mice (P < 0.01). The control group investigated the social stimulus (stranger cage)significantly longer than the object stimulus(empty cage), thereby demonstrating normal social preference. The model group showed a decrease (sniffing time and frequency)in social investigation (P < 0.05,P < 0.01, vs the control group). The chronic restraint stress group did not demonstrate social preference. The preference index in chronic restraint stress model (ratio time sniffing stranger vs. empty cage) was significantly lower compared with the control mice (P < 0.01). Conclusion The chronic restraint stress showed social avoidance in sociability test.