槐白皮对放射损伤人鼠骨髓VEGF、G-CSF的表达及超微结构的影响

目的:通过检测急性放射损伤小鼠骨髓组织中血管内皮细胞生长因子(VEGF)、粒细胞集落刺激因子(G-CSF)的表达及骨髓细胞超微结构的变化,探讨槐白皮对骨髓造血细胞和造血微环境的影响.方法:实验随机分为5组,即正常、照射对照和槐白皮高、中、低剂量(0.8、0.4、0.2mL·kg-1)组.采用免疫细胞化学SABC染色法检...

Effect of Sphora japonica on the Expression of VEGF and G-CSF in Bone Marrow Tissues and Ultra-structure of Bone Marrow in Radiation Injured Mice

OBJECTIVE：To determine the expression of vascular endothelial growth factor（VEGF）,granulocyte colony-stimulating factor（G-CSF） and ultra-structure of bone marrow in mice with acute radiation injury,in order to evaluate the effect of Sphora japonica on the hematopoietic microenvironment and hematopoietic cells of bone marrow.METHODS：40 mice were randomly divided into 5 groups：normal group,irradiation control group,S.japonica high-dose,medium-dose and low-dose groups（0.8 mL·kg-1,0.4 mL·kg-1 and 0.2 mL·kg-1）.The expressions of VEGF and G-CSF in bone marrow tissue were determined by immunocytochemistry SABC staining.Transmission electron microscope was used to observe the change of ultra-structure of bone marrow cells.RESULTS：The expression of VEGF in bone marrow tissue in S.japonica high-dose,medium-dose and low-dose groups was significantly higher than in irradiation control group（P0.05）.The expression of G-CSF in S.japonica medium-dose group was significantly higher than in irradiation control group（P0.01）,but there was no significant difference in S.japonica high-dose and low-dose groups（P0.05）.There was no significant different in the expression of VEGF and G-CSF between S.japonica medium-dose group and normal group（P0.05）,but the expression of VEGF and G-CSF in S.japonica high-dose and low-dose groups was lower than normal group（P0.05）.The ultra-structure of bone marrow showed bone marrow cells arranged regularly and closely,chromatin was uniform,pyknotic nuclear was not found,rough endoplasmic reticulum was abundant but expanded mildly.CONCLUSIONS：S.japonica can improve the hematopoietic microenvironment in mice with acute radiation injury and promote the growth of hematopoietic cells.