UV法与HPLC法测定青蒿中青蒿素含量的比较

目的:分别以高效液相色谱(HPLC)法与紫外分光光度(UV)法测定青蒿中青蒿素的含量,比较2种方法分析结果的差异。方法:HPLC法中色谱柱为Kromasil ODS C18(250mm×4.6mm,5μm),流动相为甲醇-水(75:25),流速为1.0mL·min-1,检测波长为203nm,柱温为30℃。UV法中样品溶液用0.2%NaOH衍生后,在292nm波长处测定。结果:HPLC法标准曲线的线性范围为0.025～0.400mg·mL-1(r=0.9999),平均加样回收率为97.18%(RSD=3.58%,n=9);UV法标准曲线的线性范围为4～20μg·mL-1(r=0.9998)。UV法含量测定结果比HPLC法平均高出2.05倍。结论:HPLC法的精密度、稳定性、加样回收率都较好,对青蒿中青蒿素含量的分析较为准确。UV法不宜单独用于青蒿中青蒿素的含量分析。

Content Determination of Artemisinin in Artemisia annua by HPLC vs.UV Method

OBJECTIVE：To determine the content of artemisinin in Artemisia annua by HPLC vs.UV method,and to compare the results of HPLC and UV method.METHODS：The separation was performed on Kromasil ODS C18 column（250 mm×4.6 mm,5μm）.The mobile phase consisted of methanol-water（75：25）at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 203 nm and column temperature was 30℃.The detection wavelength was at 292 nm after sample solution had been derived with 0.2% NaOH.RESULTS：The linear rang of artemisinin was 0.025～0.400 mg·mL-1（r=0.999 9）by HPLC Method.The average recovery was 97.18%（RSD=3.58%,n=9）.The linear rang of artemisinin was 4～20μg·mL-1（r=0.999 8）by UV method.The result of UV method was 2.05 times higher than the results of HPLC.CONCLUSION：The precision,stability and recovery rate of HPLC are satisfied.The content determination of artemisinin in A.annua is accurate relatively.UV method is not appropriate for the determination of artemisinin in A.annua.