HPLC法测定接骨七厘片中血竭素的含量

目的:建立测定接骨七厘片中血竭素含量的方法。方法:采用高效液相色谱法。色谱柱为Shim-pack VP-ODS柱,流动相为乙腈-0.05%磷酸二氢钠溶液(43∶57),柱温为40℃,流速为1.0mL·min-1,检测波长为440nm,进样量为10μL。结果:血竭素进样量在0.046～0.230μg范围内与峰面积积分值线性关系良好(r=0.9999);平均加样回收率为97.64%,RSD=1.52%(n=6)。结论:该方法专属性强、灵敏度高、重复性好,可用于接骨七厘片的质量控制。

Content Determination of Sanguis Draconis in Jiegu Qili Tablets by HPLC

To establish an method for determining sanguis draconis contents in Jiegu qili tablets. METHODS： The HPLC method was adopted. The determination was performed on Shim-pack VP-ODS column with mobile phase consisted of acetonitrile-0.05% sodium dihydrogen phosphate solution （43：57） at 40 ℃. The flow rate was 1.0 mL·min^-1 with UV detection wavelength at 440 nm. The injection volumn was 10 μL. RESULTS： The linear range of sanguis draeonis was 0.046-0.230 μg （r= 0.999 9） with an average recovery of 97.64% （RSD= 1.52%, n=6）. CONCLUSION： The method is specific, sensitive and reproducible. It can be used for quality control of Jiegu qili tablets.