HPLC法测定如意金黄散中5种活性成分的含量

目的:建立同时测定如意金黄散中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚含量的方法。方法:采用高效液相色谱法。色谱柱为Shimadzu C18柱,流动相为乙腈-0.1%磷酸(梯度洗脱),流速为1.0mL·min-1,检测波长为254nm,柱温为25℃,进样量为20μL。结果:5种成分的进样量分别在0.042～0.672μg(r=0.9994)、0.1676～2.6816μg(r=0.9999)、0.084～1.344μg(r=0.9996)、0.0926～1.4816μg(r=0.9998)和0.1744～2.7904μg(r=0.9991)范围内与各自峰面积积分值呈良好的线性关系;平均回收率分别为97.95%(RSD=0.88%)、97.59%(RSD=1.20%)、98.25%(RSD=1.79%)、97.93%(RSD=0.57%)和98.03%(RSD=1.33%)。结论:本方法简便、准确,可同时测定如意金黄散中5种活性成分的含量。

Determination of 5 Active Components in Ruyi Jinhuang Power by HPLC

OBJECTIVE：To establish a method for the simultaneous determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Ruyi jinhuang power.METHODS：HPLC method was adopted.The sample was separated on a Shimadzu C18 column with acetonitrile-0.1% phosphoric acid as mobile phase（gradient elution） at flow rate of 1.0 mL·min-1.The detection wavelength was set at 254 nm and the column temperature was 25 ℃.The injection volume was 20 μL.RESULTS：Aloe-emodin,rhein,emodin,chrysophanol and physcion had good linear relationship in the range of 0.042～0.672 μg（r=0.999 4）,0.167 6～2.681 6 μg（r=0.999 9）,0.084～1.344 μg（r=0.999 6）,0.092 6～1.481 6 μg（r=0.999 8）and 0.174 4～2.790 4 μg（r=0.999 1）.Their average recoveries were 97.95%（RSD=0.88%）,97.59%（RSD=1.20%）,98.25%（RSD=1.79%）,97.93%（RSD=0.57%）and 98.03%（RSD=1.33%）,respectively.CONCLUSION：The method is simple,accurate and suitable for the content determination of 5 active components of Ruyi jinhuang power simultaneously.