GC法测定万金油中桉油精和丁香酚的含量

目的:建立万金油中桉油精和丁香酚的含量测定方法。方法:采用气相色谱法。色谱柱为HP-FFAP,固定相为交联聚乙二醇-TPA;氢火焰离子化检测器,进样口温度:230℃,检测器温度:250℃;分流进样,分流比:20∶1;程序升温:初始80℃,保持3min,以6℃·min-1升至200℃,保持7min;载气:氮气,流速:1.0mL·min-1。结果:桉油精和丁香酚检测浓度线性范围分别为0.091～1.174(r=0.9999)、0.014～0.174mg·mL-1(r=0.9981),平均回收率分别为100.49%(RSD=1.47%,n=6)和100.01%(RSD=1.82%,n=6)。结论:该方法简便、快速、准确,可用于万金油的质量控制。

Content Determination of Cineol and Eugenol in Tiger Balm by GC

OBJECTIVE： To establish the method for the content determination of cineol and eugenol in tiger balm. METHODS： GC was adopted. The determination was performed on HP-FFAP column using PEG-TPA as stationary phase. Hydrogen flame ionization detector was specified with inlet temperature of 230 ℃ by split sampling. The temperature of detector was 250 ℃, and rate of split flow was 20 ： 1. The increase of culumn temperature was controlled by programming. The initial temperature was 80℃ and maintained for 3 min, and then raised to 200℃ at rate of 6 ℃. min t for 7 min. Nitrogen was used as carrier and the flow rate was 1.0 mL.min^-1. RESULTS： The linear ranges were 0.091-1.174 mg.mL^-1 （r=0.999 9） for cineol and 0.014-0.174 mg.mL^-1 （r=0.998 1） for eugenol. The average recoveries were 100.49% （RSD= 1.47%, n=6）and 100.01% （RSD= 1.82%, n= 6） respectively. CONCLUSION： The method is simple, rapid and accurate for quality control of tiger balm.