黄芪饮片中4个异黄酮类成分的含量测定

摘 要 目的:建立高效液相色谱法测定黄芪饮片中4个异黄酮成分的含量方法。方法: 色谱柱为Agilent Zorbax SB-C₁₈(250 mm×4.6 mm，5 μm) ，流动相为乙腈-水梯度洗脱，流速1.0 ml·min^-1，检测波长为260 nm, 柱温30℃，进样量10 μl。结果:毛蕊异黄酮-7-葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素分别在4.42～442 μg·ml^-1、2.06～206 μg·ml^-1、3.36～336 μg·ml^-1、4.60～460 μg·ml^-1浓度范围内与各自峰面积积分值呈良好的线性关系；平均回收率分别为99.4%(RSD=5.34%)、94.8%(RSD=2.39%)、98.9%(RSD=3.69%)、102.3%(RSD=3.01%)(n=9)。结论:本方法简便、准确、重复性好，可用于黄芪饮片的质量控制。

Simultaneous Determination of Four Isoflavonoids in Radix Astragali by HPLC

Objective：To establish an HPLC method for the determination of four isoflavonoids in Radix Astragali from 6 different batches.Method：An Agilent Zorbax ODS column was used with gradient elution composed of acetonitrile and water at a flow rate of 1.0 ml·lml^-1.The detection wavelength was set at 260 nm.Result：The linear range was 4.42-442.00μg -ml^-1 for calycosin-7-oglucoside, 2.06-206.00μg·lml^-1 for ononin,3.36-336.00μg·lml^-1 for calycosin and 4.60-460.00μg·lml^-1 for formononetin.The average recovery was 99.4%（RSD=5.34%）,94.8%（RSD = 2.39%）,98.9%（RSD = 3.69%） and 102.3%（RSD = 3.01%）, respectively（n =9）.Conclusion：The method is simple,accurate and reproducible for the quality control of Astragali Radix.