大豆苷元对氨茶碱在大鼠体内药动学的影响

摘 要 目的:研究大豆苷元对氨茶碱在大鼠体内药动学的影响。方法: 采用HPLC方法测定大豆苷元和氨茶碱合并给药组与氨茶碱单独给药组茶碱在大鼠体内的血药浓度，比较两者的药动学参数。结果:①茶碱在0.2～20.0 μg·ml^-1浓度范围内线性关系良好，定量下限为0.2μg·ml^-1，低中高3个浓度的绝对回收率分别为（86.7±4.2）%、（90.5±3.4）%和（92.4±4.6）%，相对回收率均大于90%，日间和日内精密度RSD分别小于8.94%、9.01%；②大豆苷元和氨茶碱合并给药组和单独给药组药动学参数分别为：半衰期（t_{1/2）为(123.63±18.23)和(133.94±11.20)min，曲线下面积（AUC(0-∞)）为(1 861.03±511.23)和(2 075.41±720.96) μg·min·ml^-1，AUC(0-8)为（1 749.71±376.68）和（1 963.34±475.84）μg·min·ml^-1，达峰浓度Cmax为(10.35±0.95)和(10.23±0.82)μg·ml^-1；③合并给药组较单独给药组的主要药动学参数峰值Cmax相似，t1/2、AUC有一定降低，但差异无统计学意义。结论:大豆苷元对氨茶碱在大鼠体内的药动学无明显影响。}

Effects of Daidzein on the Pharmacokinetics of Aminophylline in Rats

Objective： To study the effect of daidzein on the pharmacokinetics of aminophylline in rats. Method： The rats were divided into two groups： one was given aminophylline and the other was given the combination of daidzein and aminophylline. Plasma concentrations of the two groups were detected by HPLC. The pharmacokinetic parameters were calculated and compared by statistical analysis. Result： Excellent linear relationship of theophylline was obtained within the range of 0.2-20.0 μg·ml^-1 with LOQ of 0.2 μg·ml^-1. The absolute recovery of low, medium and high concentration was （ 86.7 ± 4.2 ） %, （90.5 ± 3.4 ） % and （ 92.4 ± 4.6）%, respectively. The relative recovery was above 90%. The inter-day RSD was below 8.94% and intar-day RSD was below 9.01%. The pharmacokinetic parameters of aminophylline in the combination group and the individual group were as follows ： t1/2 of （ 123.63 ± 18.23 ） and （ 133.94 ± 11.20 ） min, AUC（0-∞） of （ 1861.03 ± 511.23 ） and （ 2075.41 ± 720.96 ） μg · min · ml^-1, AUC（0-8） of （1 749.71 ±376.68） and （1 963.34 ±475.84）μg ·min ·ml^-1, Cmax of （10.35 ±0.95） and （10.23 ±0.82）μg ml^-1. There were no significant differences in the main pharmacokinetic parameters （C AUC and t1/2 ） between the two groups. Conclusion： Daidzein shows little influence on the pharmacokinetics of aminophylline in rats.