| 丰城鸡血藤中刺芒柄花素的大鼠肠吸收研究 |
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| 引用本文： | 刘亚丽,熊贤兵,苏丹,宋永贵,张凌,杨世林. 丰城鸡血藤中刺芒柄花素的大鼠肠吸收研究[J]. 中国中药杂志, 2013, 38(20): 3571-3575 |
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| 作者姓名： | 刘亚丽 熊贤兵 苏丹 宋永贵 张凌 杨世林 |
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| 作者单位： | 江西中医学院 中药固体制剂制造技术国家工程研究中心, 江西 南昌 330006;苏州大学 药学院, 江苏 苏州 215123;解放军第九四医院 药械科, 江西 南昌 330002;江西中医学院 中药固体制剂制造技术国家工程研究中心, 江西 南昌 330006;江西中医学院 中药固体制剂制造技术国家工程研究中心, 江西 南昌 330006;江西中医学院 中药固体制剂制造技术国家工程研究中心, 江西 南昌 330006;江西中医学院 中药固体制剂制造技术国家工程研究中心, 江西 南昌 330006 |
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| 基金项目： | 江西中医学院青年教师基金项目;江西中医学院校级课题项目 |
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| 摘 要： | 利用大鼠在体单向肠灌流模型,采用HPLC测定灌流液中刺芒柄花素含量,分别考察刺芒柄花素质量浓度、不同肠段以及P-糖蛋白抑制剂对刺芒柄花素肠吸收的影响,得出丰城鸡血藤中刺芒柄花素在大鼠体内的肠吸收机制。实验结果显示灌流液中刺芒柄花素质量浓度对吸收速率常数(Ka)和表观吸收系数(Papp)均无显著性影响;刺芒柄花素在小肠段(十二指肠、空肠和回肠)的Ka和Papp无显著性差异显著,但其Ka显著大于在结肠处的值(P<0.05),小肠段和结肠段的Papp无显著性差异;P-糖蛋白抑制剂维拉帕米对刺芒柄花素在各肠段的Ka和Papp均有显著性差异(P<0.05)。表明刺芒柄花素在大鼠肠道内的吸收机制为被动扩散,不存在饱和吸收;其在全肠段的吸收较好,吸收窗主要在小肠,且小肠内无明显的特定吸收部位;刺芒柄花素可能是P-糖蛋白的底物。
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| 关 键 词： | 刺芒柄花素 单向肠灌流 重量法 吸收速率常数 表观吸收系数 |
| 收稿时间： | 2013-03-08 |
Study on intestinal absorption of formononetin in Millettia nitita var. hirsutissima in rats |
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| LIU Ya-li,XIONG Xian-bing,SU Dan,SONG Yong-gui,ZHANG Ling and YANG Shi-lin. Study on intestinal absorption of formononetin in Millettia nitita var. hirsutissima in rats[J]. China Journal of Chinese Materia Medica, 2013, 38(20): 3571-3575 |
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| Authors: | LIU Ya-li XIONG Xian-bing SU Dan SONG Yong-gui ZHANG Ling YANG Shi-lin |
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| Affiliation: | National Engineering Center for Solid Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China;College of Pharmacy, Suzhou University, Suzhou 215123, China;Department of Medical Instrument, No.94 Hospital of PLA, Nanchang 330002, China;National Engineering Center for Solid Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China;National Engineering Center for Solid Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China;National Engineering Center for Solid Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China;National Engineering Center for Solid Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China |
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| Abstract: | To use the single-pass intestine perfusion (SPIP) model and HPLC to determine the concentration of formononetin, the effect of quality concentrations of formononetin, different intestinal segments and P-glycoprotein inhibitor on intestinal absorption of formononetin, in order to observe the intestinal absorption mechanism of formononetin from Millettia nitita var. hirsutissima in rats. The experimental results showed that the qulaity concentration of formononetin in the perfusate had no significant effect on the absorption rate constant (Ka) and the apparent absorption coefficient (Papp); Ka and Papp of formononetin in duodenum, jejunum and ileum showed no significant difference. However, Ka was significantly higher than that in colon (P<0.05), with significant difference between that in intestinum tenue and colon. P-glycoprotein inhibitor verapamil showed significant difference in Ka and Papp in intestinal segments (P<0.05). This indicated that the absorption mechanism of formononein in rat intestinal tracts passive diffusion, without any saturated absorption. Formononein is absorbed well in all intestines. Their absorption windows were mainly concentrated in the intestinum tenue, without specific absorption sites. Formononein may be the substrate of P-glycoprotein. |
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| Keywords: | formononein single-pass intestine perfusion gravimetric method absorption rate constant apparent absorption coefficient |
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