| 体外培育牛黄镇痛作用的电生理机制 |
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| 引用本文： | 张韶辉,张延琳,王文权,曹雪红,赵立波,王嘉陵.体外培育牛黄镇痛作用的电生理机制[J].中国药理学通报,2008,24(8). |
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| 作者姓名： | 张韶辉 张延琳 王文权 曹雪红 赵立波 王嘉陵 |
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| 作者单位： | 1. 华中科技大学,同济医学院药理学系,湖北,武汉,430030
2. 华中科技大学,同济医院口腔医学中心,湖北,武汉,430030 |
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| 摘 要： | 目的探讨体外培育牛黄(calculus bovis sativus,CBS)镇痛作用的电生理机制。方法在蟾蜍坐骨神经干和培养大鼠三叉神经节(TRG)细胞上,分别记录复合动作电位和电压依赖性钠通道电流。结果CBS(>0.4g.L-1)可以抑制复合神经动作电位,4g.L-1CBS对其抑制率为(42.5±17.6)%。CBS(0.2~20mg.L-1)可以剂量依赖性的抑制大鼠TRG细胞电压依赖性总钠通道电流(INa-T)和TTX不敏感型钠通道电流(INa-TTX-r),20mg.L-1CBS对INa-T和INa-TTX-r的抑制率分别为(55.8±7.8)%和(39.3±5.7)%。结论CBS对钠电流的阻滞作用可能是其镇痛作用机制之一。
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| 关 键 词： | 体外培育牛黄 膜片钳 钠离子通道 三叉神经元 |
The electrophysiological mechanism of relieving pain of calculus bovis sativus |
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| ZHANG Shao-hui,ZHANG Yan-lin,WANG Wen-quan,CAO Xue-hong,ZHAO Li-bo,WANG Jia-ling.The electrophysiological mechanism of relieving pain of calculus bovis sativus[J].Chinese Pharmacological Bulletin,2008,24(8). |
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| Authors: | ZHANG Shao-hui ZHANG Yan-lin WANG Wen-quan CAO Xue-hong ZHAO Li-bo WANG Jia-ling |
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| Abstract: | Aim To investigate the electrophysiological mechanism of relieving pain of calculus bovis sativus(CBS).Methods The action potential and the currents of voltage-dependent sodium channel were respectively recorded with RM6240BD polygraph and PC2C resistor-feedback amplifier.Results CBS(>0.4 g·L-1)decreased the action potential on sciatic nerve trunk of toads,4 g·L-1 CBS inhibited the am-plitude of action potential by(42.5±17.6)%.Furthermore,CBS(0.2~20 mg·L-1)could concentration-dependently inhibit INa-T and INa-TTX-r of cultured adult rat trigeminal ganglion neurons,and 20 mg·L-1 CBS inhibited INa-T and INa-TTX-r by(55.8±7.8)% and(39.3±5.7)%.Conclusion The inhibitory effect of CBS on the sodium current might contribute to its function of relieving pain. |
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| Keywords: | calculus bovis sativus patch clamp sodium channel trigeminal ganglion |
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