| RP—HPLC法测定肠愈颗粒中人参皂苷Rg1的含量 |
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| 引用本文： | 周风云,潘林梅.RP—HPLC法测定肠愈颗粒中人参皂苷Rg1的含量[J].中国中医药信息杂志,2009,16(7):43-44. |
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| 作者姓名： | 周风云 潘林梅 |
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| 作者单位： | [1]江阴市中医院,江苏江阴214400 [2]南京中医药大学,江苏南京210029 |
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| 摘 要： | 目的建立肠愈颗粒中人参皂苷Rg1的含量测定方法。方法采用反相高效液相色谱法，色谱柱：C18（4．6 mm×250mm，5μm）；流动相：乙腈-水（80：20）；流速：1．0mL／min；检测波长为210nm。结果肠愈颗粒中人参皂苷Rg1的线性范围为0．125～4μg，=0．9997，平均回收率为97．30％，RSD=1．31％。结论建立的方法简便可靠，重复性较好，提高了肠愈颗粒质量控制方法。
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| 关 键 词： | 肠愈颗粒 人参皂苷Rg1 反相高效液相色谱法 含量测定 |
Determination of Ginsenoside Rg1 in Changyu Granules by RP-HPLC |
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| Institution: | ZHOU Feng-yun, PAN Lin-mei(1.Jiangyin Hospital of TCM, Jiangyin 214400, China： 2.Nanjing University of TCM, Nanjing 210029, China) |
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| Abstract: | Objective To set up a method for the determination of ginsenoside Rg1 in Changyu Granules. Methods The content of ginsenoside Rg1 was determined by RP-HPLC on C18 column （4.6 min×250 mm, 5μm）. The mobile phase consisted of ethane nitrile and water （80 ： 20）. The flow rate was 1.0 mL/min. The detection wavelength was at 210 nm. Results Ginsenoside Rg1 showed a good linear relationship at the range of 0.125-4μg, r=0.999 7. The average recovery rate was 97.30%, RSD=1.31%. Conclusion The method is simple with good reproducibility, and can be used for the quality control of Changvu Granules. |
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| Keywords: | Changyu Granules ginsenoside Rg1 RP-HPLC content determination |
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