Role of Matrix Metalloproteinase- 1 (MMP- 1)/Protease-activated Receptor-1 (PAR-1)Signaling Pathway in the Cervical Cancer Invasion |
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作者姓名: | Ning-xia SUN Qian ZHAO Chen YE Yan MA Wen LI |
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作者单位: | Reproductive Center, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China |
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基金项目: | This research was supported by the project of Shanghai Municipal Health Bureau (2011160) |
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摘 要: | Objective To investigate the role of matrix metalloproteinase-1 (MMP-1)/protease- activated receptor-1 (PAR-l) signaling in the cervical cancer invasion. Methods RT-PCR was used to test the mRNA level of MMP-1 in 59 cases of cervical squamous cell cancer. Cell invasion was evaluated by Transwell assay to explore the effect of adding human recombinant MMP-1 (rhMMP-1) and PARI-siRNA on cervical cancer invasion. Results In cervical cancer tissues, more MMP-1 expression was observed than that in the normal cervical tissues, and its expression correlated with cancer status. Human recombinant MMP-1 (rhMMP-1) could promote Hela cell invasion, and its number of invasive cell correlated with the concentration of rhMMP-1. Disrupting the expression of PAR-1 reduced the MMP-1 promoting-effect on Hela cell invasion, but had no effect on non-MMP-1 invasive action. Conclusion The MMP-1/PAR-1 signaling pathway is involved in cervical cancer invasion. Therefore, blocking PAR-1 may represent a new therapeutic option for metastatic cervical cancer.
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关 键 词: | 蛋白酶激活受体 基质金属蛋白酶 MMP-1 宫颈癌 入侵途径 信令 RT-PCR方法 Hela细胞 |
收稿时间: | 5 January 2014 |
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