| Transfection of the glial cell line-derived neurotrophic factor gene promotes neuronal differentiation简 |
| |
| 作者姓名： | Jie Du Xiaoqing Gao Li Deng Nengbin Chang Huailin Xiong Yu Zheng |
| |
| 摘 要： | Glial cell line-derived neurotrophic factor recombinant adenovirus vector-transfected bone marrow mesenchymal stem cells were induced to differentiate into neuron-like cells using inductive medium containing retinoic acid and epidermal growth factor. Cell viability, micro- tubule-associated protein 2-positive cell ratio, and the expression levels of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 protein in the su- pernatant were significantly higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mes- enchymal stem cells. Furthermore, microtubule-associated protein 2, glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein743 mRNA levels in cell pellets were statistically higher in glial cell line-derived neurotrophic factor/bone marrow mesen- chymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. These results suggest that glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells have a higher rate of induction into neuron-like cells, and this enhanced differentiation into neuron-like cells may be associated with up-regulated expression of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43.
|
| 关 键 词： | 胶质细胞源性神经营养因子 胶质细胞系源性神经营养因子 神经细胞 基因转染 骨髓间充质干细胞 分化 重组腺病毒载体 神经胶质细胞 |
Transfection of the glial cell line-derived neurotrophic factor gene promotes neuronal differentiation |
| |
| Jie Du,Xiaoqing Gao,Li Deng,Nengbin Chang,Huailin Xiong,Yu Zheng.Transfection of the glial cell line-derived neurotrophic factor gene promotes neuronal differentiation[J].Neural Regeneration Research,2014,9(1):33-40. |
| |
| Authors: | Jie Du Xiaoqing Gao Li Deng Nengbin Chang Huailin Xiong Yu Zheng |
| |
| Institution: | [1]Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province, China [2]Department of Anatomy, Luzhou Medical College, Luzhou , Sichuan Province, China [3]Research Center for Preclinical Medicine, Luzhou Medical College, Luzhou , Sichuan Province, China |
| |
| Abstract: | Glial cell line-derived neurotrophic factor recombinant adenovirus vector-transfected bone marrow mesenchymal stem cells were induced to differentiate into neuron-like cells using inductive medium containing retinoic acid and epidermal growth factor. Cell viability, microtubule-associated protein 2-positive cell ratio, and the expression levels of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 protein in the supernatant were significantly higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. Furthermore, microtubule-associated protein 2, glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 mRNA levels in cell pellets were statistically higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. These results suggest that glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells have a higher rate of induction into neuron-like cells, and this enhanced differentiation into neuron-like cells may be associated with up-regulated expression of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43. |
| |
| Keywords: | nerve regeneration bone marrow mesenchymal stem cells cell differentiation neu-ron-like cells glial cell line-derived neurotrophic factor recombinant adenovirus vector transfection retinoic acid epidermal growth factor nerve growth factor growth-associated protein-43 neuralregeneration |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
