| Abstract: | A new type of well-scintillation counter with a double-well and single-plastic scintillator (DW-counter) was developed to simplify time consuming and cumbersome dilution procedures inherent to in-vivo sample measurement. It has the potential to measure many radionuclides which emit a gamma ray or positron. We tested the counting efficiency (CE) of the DW-counter (DCM-200, Aloka Co., Tokyo, Japan) with respect to 6 radionuclides. MATERIALS AND METHODS: The outline of DW-counter is altered to a single unit as compared to the prototype, while its basic mechanical constitution was not changed. Six commercially available radionuclides (Tl-201, Tc-99m, I-123, Ga-67, In-111, I-131) were used in this study. For each radionuclide, we prepared two standard solutions containing high (>100 MBq/ml) and relatively low radioactivity (10-20 MBq/ml). The radioactivity (Bq) of the radionuclide in each sample at time = 0 was measured with a dose calibrator. Afterwards, it was determined from a decay-time with correction by the physical half-life of each radionuclide. Count rate (cps) of each standard sample was measured in each well ten times per sample. The counting efficiency (CE) of the counter for each radionuclide was determined by measured count rate (cps)/standard radioactivity (Bq) x 100 (%). The conversion constant (CC) which predicts standard radioactivity (Bq) from measured count rate (cps) was obtained as a reciprocal value of the CE. RESULTS: The CE (mean +/- SD) in well-A to Tl-201, Tc-99m, I-123, Ga-67, In-111 and I-131 was 5.90 +/- 0.285%, 8.56 +/- 0.0981%, 8.33 +/- 0.344%, 7.77 +/- 0.15%, 16.4 +/- 0.495% and 10.2 +/- 0.139%, respectively. They were significantly different. The coefficient of variation of the measured count rates was less than 1% in radioactive range higher than 10(3) Bq in well-A and 106 Bq in well-B. The difference in the CE between well-A and -B ranged from 7.614 x 10(2) (I-131) to 9.395 x 10(2) (Tl). The CC ranged from 6.14 (In) to 17.15 (Tl) in well-A and from 5.05057 x 10(3) (In) to 15.83773 x 10(3) (Tl) in well-B. The CE was not significantly affected by a sample volume from 1 to 4 ml in well-A, but showed a slight difference in well-B, which seemed due to a collimation. CONCLUSION: The measurement error of the DW-counter was less than 1% and the measured count rate (cps) was exactly converted to the standard radioactivity (Bq) by determined CC. The counter is considered useful in the easy evaluation of in-vivo tracer kinetics by avoiding time consuming and cumbersome dilution techniques. |
