和络舒肝胶囊抗小鼠血吸虫性肝纤维化作用的研究

目的 观察和络舒肝胶囊对血吸虫肝纤维化的治疗作用。 方法 将84只昆明小鼠分为正常对照组、模型组及和络舒肝组。采用日本血吸虫尾蚴40条/只鼠攻击感染小鼠，6周后建立血吸虫性肝纤维化模型，用生理盐水配制的和络舒肝混悬液灌胃（2粒/20 ml生理盐水， 1 ml/只），每天1次，连续8周。免疫组化检测肝组织血管内皮生长因子（VEGF）、局部黏着斑激酶（FAK）、I型胶原和III型胶原的表达。 结果 和络舒肝胶囊能减轻血吸虫性肝纤维化的组织病理改变，降低血吸虫性肝纤维化小鼠肝组织I型胶原和III型胶原的表达，尤其是III型胶原（P<0.01）。血吸虫性肝纤维化中，VEGF和FAK的表达增加，使用和络舒肝胶囊可显著抑制肝组织中VEGF和FAK的表达（P<0.01）。 结论 和络舒肝胶囊对血吸虫性肝纤维化有治疗作用，其作用机制可能与影响肝星状细胞的活化和肝内血管病变有关。

Study on the Effect of Heluoshugan Capsule on Liver FibrosisInduced by Schistosoma japonicum Infection in Mice

Objective To investigate the effect of Chinese traditional medicine heluoshugan capsule on liver fibrosis induced by Schistosoma japonicum infection in mice. Methods Liver fibrosis in mice was established by Schistosoma japonicum infection in 6 weeks. Suspension of heluoshugan prepared with normal saline was given orally to the mice， 2 capsules for 20 mice daily for 8 weeks. The level of vascular endothelial growth factor （VEGF）、 focal adhesion kinase （FAK） and type I、 III collagen in liver tissue were detected by immuno-histochemistry. Results The results showed that heluoshugan improved the pathological change of the liver tissue， decreased the level of type I、 III collagen， espec-ially type III collagen （P<0.01）. The level of VEGF and FAK expression was inhibited after the administration of heluosh-ugan， though the level usually increased in liver fibrosis due to the infection. Conclusions The result suggests that heluoshugan capsule might have therapeutic effect on liver fibrosis induced by the infection of Schistosoma japonicum in mice， by inhibiting the activation of hepatic stellate cells and the pathological change of liver blood vessel.