抗放利对电离辐射诱导人外周血淋巴细胞遗传损伤的保护作用

目的 探讨2，2-二甲基四氢噻唑盐酸盐（抗放利）对辐射诱导人外周血淋巴细胞遗传损伤的防护作用。方法 采集健康人外周血，分为健康对照组、单纯照射组和抗放利防护组。照射剂量为2 Gy。抗放利防护组分0.2、0.5、1、2 mmol/L 4个浓度组，于照前30 min给药。通过染色体畸变分析、微核及单细胞凝胶电泳检测，观察抗放利对辐射致人外周血淋巴细胞遗传损伤的防护作用。结果 各浓度抗放利防护组与单纯照射组比较，染色体畸变率（t=5.34~25.48，P<0.05）、微核细胞率（t=5.18~29.44，P<0.05）与微核率（t=4.67~12.04，P<0.05）、彗星细胞尾长（t=16.18~19.64，P<0.05）、 尾矩（t=11.79~13.01，P<0.05）和Olive尾矩（t=12.44~14.88，P<0.05）、 尾部DNA含量（t=12.05~17.30，P<0.05）均明显降低。两个高浓度防护组（1、2 mmol/L）分别与两个低浓度防护组（0.2、0.5 mmol/L）比较，Olive尾矩明显降低（t=5.67~16.56，P<0.05）。2 mmol/L防护组与其余各防护组相比，微核率和微核细胞率明显降低（t=4.23~5.57，P<0.05）。结论 抗放利对辐射诱导人外周血淋巴细胞遗传损伤具有防护作用。

The protective effect of 2,2-dimethylthiazolidine on human peripheral blood lymphocytes in radiation-induced genetic damage

Objective To investigate the protective effect of 2,2-dimethylthiazolidine (KFL) on human peripheral blood lymphocytes in radiation-induced genetic damage. Methods Peripheral blood samples were collected from healthy volunteers and divided into normal control group, irradiation group and KFL protective groups with four different concentrations (0.2, 0.5, 1, 2 mmol/L). KFL was administrated 30 min before 2 Gy irradiation. Chromosome aberration analysis, micronucleus test and single cell gel electrophoresis were used to observe the protective effects of KFL on the radiation-induced genetic damage in human peripheral blood lymphocytes. Results Compared with the irradiation group, the four KFL protection groups showed significant reduction in the chromosomal aberration frequencies (t=5.34-25.48, P<0.05), the micronucleus cell frequencies (t=5.18-29.44, P<0.05), the micronucleus frequencies (t=4.67-12.04, P<0.05), the tailleghth (t=16.18-19.64, P<0.05), the tailmoment (t=11.79-13.01, P<0.05), the olivetailmoment (t=12.44-14.88, P<0.05)and the tail DNA% of comet cells(t=12.05-17.30, P<0.05). The olivetailmoment of the two high concentrations in protective groups(1, 2 mmol/L)were significantly decreased compared with the other two KFL groups(0.2, 0.5 mmol/L), respectively (t=5.67-16.56, P<0.05). Compared with other KFL groups, the micronucleus frequencies and micronucleus cell frequencies of the 2 mmol/L KFL group was significantly decreased (t=4.23-5.57, P<0.05). Conclusions KFL may have protective effect on human peripheral blood lymphocytes in the genetic damage induced by radiation.