实时荧光定量PCR法检测乙型肝炎患者血清HBVDNA含量512例分析

目的探讨实时荧光定量PCR检测不同血清标志物模式的乙型肝炎患者的血清HBVDNA含量的特点。方法应用ELISA对512例病人血清中乙型肝炎血清标志物进行检测，同时用实时荧光定量PCR检测标本中HBVDNA的含量，分别统计每组的HBVDNA阳性例数及其阳性率，用u检验进行统计学处理。结果HBsAg（＋）／HBeAg（＋）／抗HBc㈩组168例，HBVDNA阳性161例，占95．8％；HBsAg（＋）／抗HBe（＋）／抗HBc（＋）组163例，HBVDNA阳性51例，占31．3％；HBsAg（＋）／抗HBc（＋）组181例，HBVDNA阳性94例占51．9％；三组之间互相比较差异均有显著性（P〈0．001）。结论HBsAg（＋）／HBeAg（＋）／抗HBc（＋）组中HBV复制最活跃，实时荧光定量PCR检测HBVDNA比血清标志更能反映乙型肝炎病毒存在与复制情况。

Use the methods of Real-time fluorescence quantitative PCR to detect the HBV DNA content in the patients serum froth 312 cases of the hepatitis B pstients

Objective To investigate the characteristics of serum HBV DNA content through detecting the diffent serum markers of hepatitis B using the method of the real-time fluorescence quantitative PCR.Methods 512 cases of hepatitis B serum markers were detected with ELISA, and simultaneously use the real-time fluorescence quantitative PCR to detect HBV DNA content in the serum. Statistics for each group of HBV DNA-positive cases and its positive rate (%) were separately done.u test were used for the statistical treannent.Results HBsAg (+) / HBeAg (+) / anti-HBc (+) group has 168 cases, HBV DNA positive has 61 cases (95.8%); HBsAg (+) /anti-HBe (+) /anti-HBc (+) group has 163 cases,HBV DNA positive has 51 cases (31.3%); HBsAg (+) / anti-HBc (+) group has 181 cases,HBV DNA positive has 94 cases (51.9%). Comparisons among three.groups were significantly different (P<0.001).Conclusions The reproduction of HBV in the HBsAg (+)/ HBeAg (+)/ anti-HBc (+) group were the most active.Real-time fluorescence quantitative PCR detection of HBV DNA is the better method to reflect the existence and the reproduction of hepatitis B virus than Serological markers.