急性脑梗死患者血浆及脑脊液t-PA及PAI-1含量测定及其临床意义

采用双抗体夹心固相酶联免疫吸附法（ELISA）检测35例急性服梗死患者血浆和其中31例患者脑脊液t－PA及PAI-1抗原含量，与35例对照组血浆和其中20例对照组脑脊液进行比较。结果：急性脑梗死组血浆t－PA含量高于对照组，PAI-I含量显著高于对照组；其脑脊液t－PA、PAI－1含量均显著高于对照组；脑脊液中t－PA、PAI－1的含量分别与血浆中t－PA、PAI－1含量呈正相关，认为急性脑梗死患者纤溶活性明显下降；t－PAE及PAI－1参与了脑梗死之病理过程；t－PA及PAI－1抗原含量是反映体内纤溶活性的两个重要指标，可用血浆或脊液t－PA、PAI－1的含量作为判断病情的参考指标之一。

A study on the concentrations of plasma and cerebrospinal fluid t-PA and PAI-1 antigen in patients with acute cerebral infarction and their clinical significance

Plasma concentrations of tissue plasminogen activator (t PA) and plasminogen activator inhibitor 1 (PAI 1) antigen were determined in 35 patients with acute cerebral infarction and in 35 normal control subjects, cerebrospinal fluid concentrations of t PA and PAI 1 antigen were also determined in 31 patients with acute cerebral infarction and in 20 normal control subjects. The results showed that, in acute cerebral infarction group, the plasma concentrations of t PA antigen in patients were higher than those of normal control subjects (P<0.05); the plasma concentrations of PAI 1 antigen were significantly higher (P<0.01). The cerebrospinal fluid concentrations of t PA and PAI 1 antigen in the patients with acute cerebral infartion were significantly higher than those of normal control subjects; They were positively correlated with plasma concentrations of t PA and PAI 1 antigen.The neurological defect scores in patients with acute cerebral thrombosis were significantly positively correlated with plasma concentrations of t PA and PAI antigen.The fibrinolytic activity in the patients with acute cerebral thrombosis was significantly lower than normal. It suggests that the plasma t PA and PAI 1 might play a role in the pathologic changes of cerebral infarction. t PA and PAI 1 may be two important indexes of reflecting fibrinolytic activity.