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泌尿生殖道沙眼衣原体分离培养方法探讨
引用本文:朱道银 朱汉林. 泌尿生殖道沙眼衣原体分离培养方法探讨[J]. 重庆医科大学学报, 1993, 18(3): 229-232
作者姓名:朱道银 朱汉林
作者单位:重庆医科大学微生物学教研室(朱道银,朱汉林,李伟,王毅,秦亚恬),重庆医科大学微生物学教研室(裴润芳)
摘    要:

关 键 词:沙眼衣原体 感染 泌尿生殖系统

A STUDY OF METHODS FOR CULTIVATION AND ISOLATION OF GHLAMYDIA TRACHOMATlS IN GENITOURINARY TRACT
Zhu Daoyin,et al Dept of Microbiology. A STUDY OF METHODS FOR CULTIVATION AND ISOLATION OF GHLAMYDIA TRACHOMATlS IN GENITOURINARY TRACT[J]. Journal of Chongqing Medical University, 1993, 18(3): 229-232
Authors:Zhu Daoyin  et al Dept of Microbiology
Abstract:Chlamydia trachomatis in genitourinary tract were cultivated and isolated by culturing McCoy cells treated with cycloheximide. Of the 450 cases of cervicitis Or inflammatory diseases,,234(52%) positive cultures were detected. 15(50%) positive cultures were detected in 30 cases of urethritis in men. It was suggested that genitourinary infacfions caused by C.trachomatis were common in our country. Of 249 positive cultures, 73(29.3%) were detected by the second passage. Cycloheximide optimal concentration in inoculation media was 1-4ng/ml. Inclusion number of the samples centrifugad at 2000g was higher than that of uncentrifuged samples. Cells treated with cycloheximide,cefltrifugation, and a second passage were necessary for cultivation and isolation of the pathogen
Keywords:C. traehomatis   C. trachomatis cultures  genitourinary infection  
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