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| 土槿乙酸诱导前列腺癌DU-145细胞周期阻滞的机制研究 | |
| 引用本文： | 买霞,徐忠伟,陈小义,曹波,徐瑞成. 土槿乙酸诱导前列腺癌DU-145细胞周期阻滞的机制研究[J]. 中国中药杂志, 2012, 37(22): 3467-3471 |
| 作者姓名： | 买霞 徐忠伟 陈小义 曹波 徐瑞成 |
| 作者单位： | 1. 中国人民武装警察部队后勤学院细胞生物学教研室,天津300162;天津市职业和环境危害生物标志物重点实验室,天津300162 2. 中国人民武装警察部队后勤学院细胞生物学教研室,天津,300162 3. 天津市职业和环境危害生物标志物重点实验室,天津300162;中国人民武装警察部队后勤学院生药学教研室,天津300162 |
| 基金项目： | 国家自然科学基金项目(30901985) |
| 摘 要： | 目的:探讨土槿乙酸(pseudolaric acid B,PLAB)对人前列腺癌细胞株DU-145细胞增殖和细胞周期的影响.方法:MTT法检测PLAB对细胞生长抑制作用；Hoechst 33342荧光染色分析细胞死亡特征；流式细胞术检测细胞周期；Real-time PCR和Western blot检测周期相关基因cyclin B1,CDK1和cyclin D1的表达变化.结果:PLAB明显抑制DU-145细胞生长,且抑制作用呈浓度-作用时间依赖性(P＜0.05),其24,48,72 h的IC50分别为4.53,2.39,2.08 μmol·L-1.5μmol·L-1PLAB处理细胞24 h,细胞出现核染色质凝集、凋亡小体等典型凋亡特征,随着PLAB浓度升高,G2/M期细胞比例明显增大并表现出时间依赖性(P＜0.05),同时伴有cyclin B1和CDK1呈高表达状态(P＜0.05),cyclin D1呈低表达状态(P＜0.05).结论:PLAB可抑制DU-145细胞生长,引起细胞G2/M期阻滞,伴随cyclin B1和CDK1呈高表达状态,可能与其调控周期相关蛋白降解有关. |
| 关 键 词： | 土槿乙酸 人前列腺癌细胞 细胞周期 cyclin B1 CDK1 cyclin D1 |
| 收稿时间： | 2012-01-04 |
Cycle arrest of prostate carcinoma DU-145 cells induced by pseudolaric acid B |
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| MAI Xi,XU Zhong-wei,CHEN Xiao-yi,CAO Bo and XU Rui-cheng. Cycle arrest of prostate carcinoma DU-145 cells induced by pseudolaric acid B[J]. China Journal of Chinese Materia Medica, 2012, 37(22): 3467-3471 | |
| Authors: | MAI Xi XU Zhong-wei CHEN Xiao-yi CAO Bo XU Rui-cheng |
| Affiliation: | Department of Cell Biology, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China;Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Tianjin 300162, China;Department of Cell Biology, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China;Department of Cell Biology, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China;Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Tianjin 300162, China;Department of Pharmacy, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China;Department of Cell Biology, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China;Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Tianjin 300162, China |
| Abstract: | |
| Keywords: | pseudolaric acid B human prostate carcinoma cell cell cycle cyclin B1 CDK1 cyclin D1 |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |