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| 丹参2-C-甲基-D-赤藓糖醇2,4-环焦磷酸合成酶(SmMCS)基因全长克隆及其生物信息学分析 | |
| 引用本文： | 高伟,程琪庆,马晓惠,何云飞,蒋超,袁媛,黄璐琦.丹参2-C-甲基-D-赤藓糖醇2,4-环焦磷酸合成酶(SmMCS)基因全长克隆及其生物信息学分析[J].中国中药杂志,2012,37(22):3365-3370. |
| 作者姓名： | 高伟 程琪庆 马晓惠 何云飞 蒋超 袁媛 黄璐琦 |
| 作者单位： | 1. 首都医科大学中医药学院,北京,100069 2. 首都医科大学中医药学院,北京100069;中国中医科学院中药研究所,北京100700 3. 中国中医科学院中药研究所,北京100700;成都中医药大学药学院,四川成都611137 4. 中国中医科学院中药研究所,北京,100700 |
| 基金项目： | 国家自然科学基金项目(30901965);全国优秀博士学位论文作者专项资金项目(201188);北京市自然科学基金项目(5102009);北京市优秀人才培养资助个人项目(2011D005018000002) |
| 摘 要： | 目的:从丹参毛状根中克隆2-C-甲基-D-赤藓糖醇2,4-环焦磷酸合成酶(SmMCS)全长基因,并进行生物信息学分析.方法:根据丹参转录组数据提供的基因片段设计特异引物,采用RACE方法克隆SmMCS全长cDNA,并对SmMCS进行蛋白结构预测、序列多重比对和构建进化树等分析；同时采用实时定量PCR检测Ag+诱导子诱导丹参毛状根不同时期的SmMCS基因转录水平.结果:克隆的SmMCS全长cDNA由988个核苷酸组成,具有完整编码框,编码234个氨基酸,蛋白相对分子质量约24.6 kDa,等电点pI 8.53；实时定量PCR结果表明该基因受Ag+诱导后表达水平在12 h时急剧上升并达到最高值.结论:从丹参毛状根中克降得到一条SmMCS全长cDNA,为进一步研究丹参酮类成分的生物合成和萜类次生代谢提供靶基因. |
| 关 键 词： | 丹参 2-C-甲基-D-赤藓糖醇2 4-环焦磷酸合成酶 cDNA末端快速扩增 生物信息学分析 |
| 收稿时间： | 2012/9/23 0:00:00 |
Cloning and bioinformatics analysis of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase gene in Salvia miltiorrhiza |
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| GAO Wei,CHENG Qi-qing,MA Xiao-hui,HE Yun-fei,JIANG Chao,YUAN Yuan and HUANG Lu-qi.Cloning and bioinformatics analysis of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase gene in Salvia miltiorrhiza[J].China Journal of Chinese Materia Medica,2012,37(22):3365-3370. | |
| Authors: | GAO Wei CHENG Qi-qing MA Xiao-hui HE Yun-fei JIANG Chao YUAN Yuan and HUANG Lu-qi |
| Institution: | School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China;School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
| Abstract: | |
| Keywords: | Salvia miltiorrhiza 2-C-methyl-D-erythritol 2 4-cyclodiphosphate synthase rapid-amplification of cDNA ends bioinformatics analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |