bFGF、IGF-Ⅰ及TGF-β1对人髁突软骨细胞增殖的影响

目的 研究胰岛素样生长因子（ｉｎｓｕｌｉｎ－ｌｉｋｅ ｇｒｏｗｔｈ ｆａｃｔｏｒ Ⅰ，ＩＧＦ－Ⅰ），碱性成纤维细胞生长因子（ｂａｓｉｃ ｆｉｂｒｏｂｌａｓｔ ｇｒｏｗｔｈ ｂＦＧＦ），转化生长因子β１（ｔｒａｎｓｆｏｒｍｉｎｇ ｇｒｏｗｔｈ ｆａｃｔｏｒ β１，ＴＧＦ－β１）单独及联合应用，对人髁突软骨细胞增殖的剂量－效应及时间－效应。方法 采用体外细胞培养技术及噻唑蓝比色法，对３种生长因子对软骨

Effects of various growth factors on human mandibular condylar cartilage cell proliferation

OBJECTIVE: To investigate the effects of transforming growth factor-beta 1 (TGF-beta 1) insulin-like growth factor I (IGF-I) and basic fibroblast growth factor(bFGF) on human mandibular condylar cartilage (MCC) cell proliferation. METHODS: Isolated human MCC cells were cultured in DMEM supplemented with 10% newborn calf serum(NCS). The second passages were used in order to avoid chondrocyte dedifferentiation. Cells were seeded at 2 x 10(4)/well on 96-well plate. After synchronization, medium was replaced by DMEM containing 0.4% NCS or 10% NCS with various growth factors, concentrations and combinations. Dose-response and time-course were studied by MTT colorimetric method. RESULTS: In 0.4% serum containing medium, bFGF stimulated the proliferation moderately, whereas TGF-beta 1 and IGF-I had less effect. In 10% NCS condition, all three growth factors had mitogenic effect and acted dose-dependently. The effects were significant after three days. Among them, bFGF was a potent mitogen(increased by 65%), IGF-I the next(24%). The effect of TGF-beta 1 (13%) might be mediated by some other factors in the serum. The synergetic effects were achieved when they were used in combination. CONCLUSION: It is suggested that optimal combination of growth factors can promote the proliferation of MCC cells significantly, this might be an ideal way in dealing with cartilage damage during pathogenesis.