Comparison of the Sensititre YeastOne dilution method with the Clinical Laboratory Standards Institute (CLSI) M27-A3 microbroth dilution reference method for determining MIC of eight antifungal agents on 102 yeast strains |
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Authors: | S. BertoutC. Dunyach,P. DrakulovskiJ. Reynes,M. Mallié |
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Affiliation: | a UMR 145 (IRD/UM1), UFR sciences pharmaceutiques et biologiques, 15, avenue Charles-Flahault, BP 14491, 34093 Montpellier cedex 5, France b Service des maladies infectieuses et tropicales, hôpital Gui-de-Chauliac, 80, avenue Augustin-Fliche, 34295 Montpellier, France |
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Abstract: | The Clinical Laboratory Standards Institute ([CLSI] formerly NCCLS) reference broth microdilution testing method (protocol M27-A3) was compared with a commercially available methods (Sensititre YeastOne®) by testing two quality control strains and 102 isolates of Candida sp. and Cryptococcus sp. against fluconazole, itraconazole, ketoconazole, posaconazole, voriconazole, flucytosin, amphotericin B and caspofungin. Minimal inhibitory concentrations (MIC) endpoints were determined after 24 h of incubation for Sensititre YeastOne® and after 24 and 48 h for CLSI microdilution method. Essential agreements between methods vary from 70.6 to 92.2%. Categorical agreements vary from 94.1% for 5FC to 72.6% for AMB. Sensititre YeastOne® reading appears to be useful for avoiding very major errors and this confirms the interest of this method for evaluating new antifungals activity in vitro. |
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Keywords: | CLSI M27-A3 Sensititre YeastOne® Antifungal susceptibility testing Candida Cryptococcus |
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