本芴醇对疟原虫DNA含量及溶酶体pH值的影响

目的：探讨本芴醇的抗疟作用机制。方法：采用流式细胞术（FCM）分析了本芴醇和氯喹对鼠伯氏疟原虫K173株DNA含量及溶酶体PH值的影响。结果：对照组疟原虫DNA含量在各时间点没有显著变化，本芴醇单次给药后，随时间推移疟原虫DNA含量逐渐减，药后2h两给药组疟原虫红内期DNA含量开始降低，到16h降到最低，但药后24hDNA含量又有回升，本芴醇单次给药后1h起疟原虫溶醇体PH值开始升高，药后3h升至最高，药后4h原虫溶酶体PH值恢复复至药前水平。对照药氯喹对疟原虫DNA含量和溶酶体PH值也有相同影响，结论：本芴醇的抗疟作用与其抑制DNA合成相关，但与其升高溶酶体PH值的关系不明确。

Effect of benflumetol on DNA content and pH value of the lysosome of Plasmodium berghei

Objective:To study the antimalarial mechanism of benflumetol (B). Methods: Flow cytometry (FCM) was used to analyze the effects of B and chloroquine (CQ) on DNA content of Plasmodium berghei and pH value of the lysosome of malarial parasites. Results: DNA content of the plasmodia not treated with any drugs was not changed in 24 hours,while benflumetol could decrease the DNA content: the DNA content began to decrease 2 h after the drug administration and reached the minimum by 16 h, but somewhat increased at 24 h after administration. The pH in the lysosome increased 1 h and restored premedication level 4 h after benflumetol administration. Chloroquine had the same effects on DNA and lysosome pH of malarial parasites.Conclusions: The antimalarial mechanism of benflumetol is directly related to its effect to inhibit the synthesis of DNA. [